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Neurochemical Research

, Volume 41, Issue 12, pp 3333–3343 | Cite as

PRDM5 Expression and Essential Role After Acute Spinal Cord Injury in Adult Rat

  • Jie Liu
  • Weijie Wu
  • Jie Hao
  • Mingchen Yu
  • Jin Liu
  • Xinlei Chen
  • Rong Qian
  • Feng ZhangEmail author
Original Paper

Abstract

PR (PRDI-BF1 and RIZ) domain proteins (PRDM) are a subfamily of the kruppel-like zinc finger gene products that modulate cellular processes such as differentiation, cell growth and apoptosis. PRDM5 is a recently identified family member that functions as a transcriptional repressor and behaves as a putative tumor suppressor in different types of cancer. However, the expression and function of PRDM5 in spinal cord injury (SCI) are still unknown. In the present study, we have performed an acute SCI model in adult rats and investigated the dynamic changes of PRDM5 expression in the spinal cord. We found that PRDM5 protein levels gradually increased, reaching a peak at day 5 and then gradually declined to a normal level at day 14 after SCI with Western blot analysis. Double immunofluorescence staining showed that PRDM5 immunoreactivity was found in neurons, astrocytes and microglia. However, the expression of PRDM5 was increased predominantly in neurons. Additionally, colocalization of PRDM5/active caspase-3 was been respectively detected in neurons. In vitro, we found that depletion of PRDM5 by short interfering RNA, obviously decreases neuronal apoptosis. In summary, this is the first description of PRDM5 expression in SCI. Our results suggested that PRDM5 might play crucial roles in CNS pathophysiology after SCI and this research will provide new drug targets for clinical treatment of SCI.

Keywords

Spinal cord injury PR domain-containing protein 5 Neuron Apoptosis 

Abbreviations

PRDM5

PR domain-containing protein 5

SCI

Spinal cord injury

CNS

Central nervous system

NeuN

Neuronal nuclear antigen

GFAP

Glial fibrillary acidic protein

siRNA

Short interfering RNA

Notes

Acknowledgments

This work was supported by Nantong University Innovation Project (YKS14011).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jie Liu
    • 1
  • Weijie Wu
    • 1
  • Jie Hao
    • 1
  • Mingchen Yu
    • 1
  • Jin Liu
    • 1
  • Xinlei Chen
    • 1
  • Rong Qian
    • 1
  • Feng Zhang
    • 1
    Email author
  1. 1.Department of OrthopaedicsAffiliated Hospital of Nantong UniversityNantongChina

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