Neurochemical Research

, Volume 41, Issue 7, pp 1774–1783 | Cite as

Differential Expression of Adenosine P1 Receptor ADORA1 and ADORA2A Associated with Glioma Development and Tumor-Associated Epilepsy

  • Jun Huang
  • Ming-Na Chen
  • Juan Du
  • Hao Liu
  • Yu-Jiao He
  • Guo-Liang Li
  • Shu-Yu Li
  • Wei-Ping Liu
  • Xiao-Yan LongEmail author
Original Paper


Level of adenosine, an endogenous astrocyte-based neuromodulator, is primarily regulated by adenosine P1 receptors. This study assessed expression of adenosine P1 receptors, ADORA1 (adenosine A1 receptor) and ADORA2A (adenosine A2a receptor) and their association with glioma development and epilepsy in glioma patients. Expression of ADORA1/ADORA2A was assessed immunohistochemically in 65 surgically removed glioma tissue and 21 peri-tumor tissues and 8 cases of normal brain tissues obtained from hematoma patients with cerebral trauma. Immunofluorescence, Western blot, and qRT-PCR were also used to verify immunohistochemical data. Adenosine P1 receptor ADORA1 and ADORA2A proteins were localized in the cell membrane and cytoplasm and ADORA1/ADORA2A immunoreactivity was significantly stronger in glioma and peri-tumor tissues that contained infiltrating tumor cells than in normal brain tissues (p < 0.05). The World Health Organization (WHO) grade III gliomas expressed even higher level of ADORA1 and ADORA2A. Western blot and qRT-PCR confirmed immunohistochemical data. Moreover, higher levels of ADORA1 and ADORA2A expression occurred in high-grade gliomas, in which incidence of epilepsy were lower (p < 0.05). In contrast, a lower level of ADORA1/ADORA2A expression was found in peri-tumor tissues with tumor cell presence from patients with epilepsy compared to patients without epilepsy (p < 0.05). The data from the current study indicates that dysregulation in ADORA1/ADORA2A expression was associated with glioma development, whereas low level of ADORA1/ADORA2A expression could increase susceptibility of tumor-associated epilepsy.


ADORA1 ADORA2A Glioma Epilepsy Immunohistochemistry 



This study was supported in part by grants from the Scientific Research Foundation of State Education Ministry for the Returned Overseas Chinese Scholars (#2013.1792) and the Department of Science and Technology of Hunan Province, China (#2013FJ4367).

Compliance with Ethical Standards

Conflict of interest

The authors declared that there is no conflict of interest in this work.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee of Xiangya Hospital of Central South University (Changsha, Hunan, China) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jun Huang
    • 1
  • Ming-Na Chen
    • 2
  • Juan Du
    • 2
  • Hao Liu
    • 2
  • Yu-Jiao He
    • 2
  • Guo-Liang Li
    • 2
  • Shu-Yu Li
    • 2
  • Wei-Ping Liu
    • 2
  • Xiao-Yan Long
    • 2
    Email author
  1. 1.Departments of Neurosurgery, Xiangya HospitalThe Central South UniversityChangshaChina
  2. 2.Departments of Neurology, Xiangya HospitalThe Central South UniversityChangshaChina

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