Skip to main content

Advertisement

SpringerLink
Log in

Taurine Protected Against the Impairments of Neural Stem Cell Differentiated Neurons Induced by Oxygen–Glucose Deprivation

  • Original Paper
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

Cell transplantation of neural stem cells (NSCs) is a promising approach for neurological recovery both structurally and functionally. However, one big obstacle is to promote differentiation of NSCs into neurons and the followed maturation. In the present study, we aimed to investigate the protective effect of taurine on the differentiation of NSCs and subsequent maturation of their neuronal lineage, when exposed to oxygen–glucose deprivation (OGD). The results suggested that taurine (5–20 mM) promoted the viability and proliferation of NSCs, and it protected against 8 h of OGD induced impairments. Furthermore, 20 mM taurine promoted NSCs to differentiate into neurons after 7 days of culture, and it also protected against the suppressive impairments of 8 h of OGD. Consistently, taurine (20 mM) promoted the neurite sprouting and outgrowth of the NSC differentiated neurons after 14 days of differentiation, which were significantly inhibited by OGD (8 h). At D21, the mushroom spines and spine density were promoted or restored by 20 mM taurine. Taken together, the enhanced viability and proliferation of NSCs, more differentiated neurons and the promoted maturation of neurons by 20 mM taurine support its therapeutic application during stem cell therapy to enhance neurological recovery. Moreover, it protected against the impairments induced by OGD, which may highlight its role for a more direct therapeutic application especially in an ischemic stroke environment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

NSCs:

Neural stem cells

OGD:

Oxygen–glucose deprivation

References

  1. Ibrayeva A, Bonaguidi MA (2015) Pushing and pulling on adult neural stem cells. Cell Stem Cell 16:451–452

    Article  CAS  PubMed  Google Scholar 

  2. Dametti S, Faravelli I, Ruggieri M, Ramirez A, Nizzardo M, Corti S (2015) Experimental advances towards neural regeneration from induced stem cells to direct in vivo reprogramming. Mol Neurobiol. doi:10.1007/s12035-015-9181-7

    PubMed  Google Scholar 

  3. Zhao Y, Xie P, Zhu XF, Cai ZY (2008) Neural stem cell transplantation and nerve growth factor promote neurological recovery in rats with ischemic stroke. Nan Fang Yi Ke Da Xue Xue Bao 28:1123–1126

    CAS  PubMed  Google Scholar 

  4. Volkov AI, Lebedev SV, Viktorov IV, Starykh EP, Savchenko EA, Grinenko NF, Lazarenko IP, Chekhonin VP (2010) Influence of the neural stem cell transplantation on the restoration of CNS functions in rats with cortical stroke. Zh Nevrol Psikhiatr Im S S Korsakova 110:64–72

    CAS  PubMed  Google Scholar 

  5. Kokaia Z, Darsalia V (2011) Neural stem cell-based therapy for ischemic stroke. Transl Stroke Res 2:272–278

    Article  PubMed  Google Scholar 

  6. Gregoire CA, Goldenstein BL, Floriddia EM, Barnabe-Heider F, Fernandes KJ (2015) Endogenous neural stem cell responses to stroke and spinal cord injury. Glia 63(8):1469–1482

    Article  PubMed  Google Scholar 

  7. Daadi MM, Hu S, Klausner J, Li Z, Sofilos M, Sun G, Wu JC, Steinberg GK (2013) Imaging neural stem cell graft-induced structural repair in stroke. Cell Transpl 22:881–892

    Article  Google Scholar 

  8. Vinny PW, Vishnu VY, Khurana D (2015) Endovascular therapy for ischemic stroke. N Engl J Med 372:2363–2364

    Article  PubMed  Google Scholar 

  9. Liu J (2013) Induced pluripotent stem cell-derived neural stem cells: new hope for stroke? Stem Cell Res Ther 4:115

    Article  PubMed  PubMed Central  Google Scholar 

  10. Sturman JA, Gaull GE (1975) Taurine in the brain and liver of the developing human and monkey. J Neurochem 25:831–835

    Article  CAS  PubMed  Google Scholar 

  11. Miller TJ, Hanson RD, Yancey PH (2000) Developmental changes in organic osmolytes in prenatal and postnatal rat tissues. Comp Biochem Physiol A Mol Integr Physiol 125:45–56

    Article  CAS  PubMed  Google Scholar 

  12. Pasantes-Morales H, Ramos-Mandujano G, Hernandez-Benitez R (2015) Taurine enhances proliferation and promotes neuronal specification of murine and human neural stem/progenitor cells. Adv Exp Med Biol 803:457–472

    Article  PubMed  Google Scholar 

  13. Peters A, Kaiserman-Abramof IR (1969) The small pyramidal neuron of the rat cerebral cortex. The synapses upon dendritic spines. Z Zellforsch Mikrosk Anat 100:487–506

    Article  CAS  PubMed  Google Scholar 

  14. Martin RL, Lloyd HG, Cowan AI (1994) The early events of oxygen and glucose deprivation: setting the scene for neuronal death? Trends Neurosci 17:251–257

    Article  CAS  PubMed  Google Scholar 

  15. Cimarosti H, Henley JM (2008) Investigating the mechanisms underlying neuronal death in ischemia using in vitro oxygen–glucose deprivation: potential involvement of protein SUMOylation. Neuroscientist 14:626–636

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. Wang Q, Zhu GH, Xie DH, Wu WJ, Hu P (2015) Taurine enhances excitability of mouse cochlear neural stem cells by selectively promoting differentiation of glutamatergic neurons over GABAergic neurons. Neurochem Res 40:924–931

    Article  CAS  PubMed  Google Scholar 

  17. Biffi E, Regalia G, Menegon A, Ferrigno G, Pedrocchi A (2013) The influence of neuronal density and maturation on network activity of hippocampal cell cultures: a methodological study. PLoS One 8:e83899

    Article  PubMed  PubMed Central  Google Scholar 

  18. Reynolds BA, Tetzlaff W, Weiss S (1992) A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 12:4565–4574

    CAS  PubMed  Google Scholar 

  19. Benowitz LI, Routtenberg A (1997) GAP-43: an intrinsic determinant of neuronal development and plasticity. Trends Neurosci 20:84–91

    Article  CAS  PubMed  Google Scholar 

  20. Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707–1710

    Article  CAS  PubMed  Google Scholar 

  21. Gwag BJ, Lobner D, Koh JY, Wie MB, Choi DW (1995) Blockade of glutamate receptors unmasks neuronal apoptosis after oxygen–glucose deprivation in vitro. Neuroscience 68:615–619

    Article  CAS  PubMed  Google Scholar 

  22. Yablonsky-Alter E, Agovic MS, Gashi E, Lidsky TI, Friedman E, Banerjee SP (2009) Cocaine challenge enhances release of neuroprotective amino acid taurine in the striatum of chronic cocaine treated rats: a microdialysis study. Brain Res Bull 79:215–218

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Xu S, He M, Zhong M, Li L, Lu Y, Zhang Y, Zhang L, Yu Z, Zhou Z (2015) The neuroprotective effects of taurine against nickel by reducing oxidative stress and maintaining mitochondrial function in cortical neurons. Neurosci Lett 590:52–57

    Article  CAS  PubMed  Google Scholar 

  24. Wang GH, Jiang ZL, Fan XJ, Zhang L, Li X, Ke KF (2007) Neuroprotective effect of taurine against focal cerebral ischemia in rats possibly mediated by activation of both GABAA and glycine receptors. Neuropharmacology 52:1199–1209

    Article  CAS  PubMed  Google Scholar 

  25. Schultheiss M, Ruschenburg H, Warga M, Schramm C, Januschowski K, Schnichels S, Biedermann T, Szurman P, Spitzer MS (2012) Neuroprotective effects of a taurine-containing irrigation solution for vitrectomy. Retina 32:1343–1349

    Article  CAS  PubMed  Google Scholar 

  26. Kumari N, Prentice H, Wu JY (2013) Taurine and its neuroprotective role. Adv Exp Med Biol 775:19–27

    Article  CAS  PubMed  Google Scholar 

  27. Hernandez-Benitez R, Ramos-Mandujano G, Pasantes-Morales H (2012) Taurine stimulates proliferation and promotes neurogenesis of mouse adult cultured neural stem/progenitor cells. Stem Cell Res 9:24–34

    Article  CAS  PubMed  Google Scholar 

  28. Hernandez-Benitez R, Pasantes-Morales H, Saldana IT, Ramos-Mandujano G (2010) Taurine stimulates proliferation of mice embryonic cultured neural progenitor cells. J Neurosci Res 88:1673–1681

    CAS  PubMed  Google Scholar 

  29. Ramos-Mandujano G, Hernandez-Benitez R, Pasantes-Morales H (2014) Multiple mechanisms mediate the taurine-induced proliferation of neural stem/progenitor cells from the subventricular zone of the adult mouse. Stem Cell Res 12:690–702

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This work was supported by National Natural Science Foundation of China Grant (31300969), Natural Science Foundation of Jiangsu Province Grant (BK20130302) and China Postdoctoral Science Foundation (2013M540461 and 2013M541717).

Authors’ Contributions

Conceived and designed the experiments: CJ. Performed the experiments: BX. Analyzed the data: HzL ZYG. Wrote the paper: BX CJ.

Author information

Authors and Affiliations

  1. School of Biology and Basic Medical Sciences, Medical College, Soochow University, 199 Ren’ai Road, Suzhou, 215123, Jiangsu, China

    Bo Xiao, Huazhen Liu, Zeyun Gu, Sining Liu & Cheng Ji

  2. Key Laboratory for Ecology and Pollution Control of Coastal Wetlands, School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng, 224051, China

    Bo Xiao

Authors
  1. Bo Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Huazhen Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zeyun Gu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sining Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cheng Ji
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cheng Ji.

Ethics declarations

Conflict of interest

The authors declare that they have no competing financial interests.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 133 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xiao, B., Liu, H., Gu, Z. et al. Taurine Protected Against the Impairments of Neural Stem Cell Differentiated Neurons Induced by Oxygen–Glucose Deprivation. Neurochem Res 40, 2348–2356 (2015). https://doi.org/10.1007/s11064-015-1726-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11064-015-1726-7

Keywords

Search

Navigation