Protective Effect of l-Theanine on Cadmium-Induced Apoptosis in PC12 Cells by Inhibiting the Mitochondria-Mediated Pathway
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l-Theanine is an amino acid derivative from green tea. The present work was aimed at the effect of l-theanine on neuron-like rat pheochromocytoma (PC12) cells stimulated with cadmium chloride. Treatment with l-theanine before cadmium exposure increased cell viability; the experiments of Annexin V/PI staining indicated that l-theanine inhibited cadmium-induced cell apoptosis. Meanwhile, l-theanine decreased ROS production and protected from cadmium-induced disruption of mitochondrial transmembrane potential. Compared with cadmium-treated cells, l-theanine could also decrease the ratio of Bax/Bcl-2, as well as the level of cleaved caspase-9, caspase-3 and poly(ADP-ribose) polymerase. Furthermore, l-theanine depresses cadmium-induced up regulation of phosphorylations of PI3K/Akt, MAPK ERK1/2, and JNK signaling. These data suggest that l-theanine pretreatment reduces severity of cadmium toxicity probably via antioxidant action. Therefore, it may be concluded that l-theanine could be exploited for prevention of cadmium-induced diseases.
Keywordsl-Theanine Cadmium Oxidative stress Mitochondria apoptosis pathway Apoptosis
Reactive oxygen species
Mitochondrial membrane potential
Glyceraldehyde phosphate dehydrogenase
Mitogen-activated protein kinases
Mammalian target of rapamycin
Extracellular signal regulated kinases
c-Jun N-terminal kinase
This work was supported by Grants from Jiangsu perspective study (BY2013001-03), National Nature Science Foundation of China (No. 31401004), and program of Natural Science Research of Jiangsu Higher education Institution of China (No. 14KJB180010).
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Conflict of interest
Follow the established procedures approved by local Ethics Committee.
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