Linalool Inhibits LPS-Induced Inflammation in BV2 Microglia Cells by Activating Nrf2
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Linalool, a natural compound of the essential oils, has been reported to have anti-inflammatory effects. This study aimed to investigate the anti-inflammatory effects and mechanism of linalool in LPS-stimulated BV2 microglia cells. BV2 microglia cells were stimulated with LPS in the presence or absence of linalool. The production of inflammatory mediators TNF-α, IL-1β, NO, and PGE2 as well as Nrf2, HO-1 expression were detected. Our results showed that linalool inhibited LPS-induced TNF-α, IL-1β, NO, and PGE2 production in a dose-dependent manner. Linalool also inhibited LPS-induced NF-κB activation. Treatment of linalool induced nuclear translocation of Nrf2 and expression of HO-1. In addition, our results showed that the anti-inflammatory effect of linalool was attenuated by transfection with Nrf2 siRNA. In conclusion, these results suggested that linalool inhibits LPS-induced inflammation in BV2 microglia cells by activating Nrf2/HO-1 signaling pathway.
KeywordsLinalool LPS Inflammatory mediators Nrf2
Conflict of interest
All authors declare that they have no conflict of interest.
- 3.Wang S, Jing H, Yang H, Liu Z, Guo H, Chai L, Hu L (2015) Tanshinone I selectively suppresses pro-inflammatory genes expression in activated microglia and prevents nigrostriatal dopaminergic neurodegeneration in a mouse model of Parkinsons disease. J Ethnopharmacol 164:247–255PubMedCrossRefGoogle Scholar
- 11.Jayasooriya RG, Lee KT, Kang CH, Dilshara MG, Lee HJ, Choi YH, Choi IW, Kim GY (2014) Isobutyrylshikonin inhibits lipopolysaccharide-induced nitric oxide and prostaglandin E2 production in BV2 microglial cells by suppressing the PI3K/Akt-mediated nuclear transcription factor-κB pathway. Nutr Res 34:1111–1119PubMedCrossRefGoogle Scholar
- 17.Kim AR, Lee MS, Choi JW, Utsuki T, Kim JI, Jang BC, Kim HR (2013) Phlorofucofuroeckol A suppresses expression of inducible nitric oxide synthase, cyclooxygenase-2, and pro-inflammatory cytokines via inhibition of nuclear factor-κB, c-Jun NH2-terminal kinases, and Akt in microglial cells. Inflammation 36:259–271PubMedCrossRefGoogle Scholar
- 22.Zhao ZZ, Tang XF, Zhao XH, Zhang MH, Zhang WJ, Hou SH, Yuan WF, Zhang HF, Shi LJ, Jia H, Liang L, Lai Z, Gao JF, Zhang KY, Fu L, Chen W (2014) Tylvalosin exhibits anti-inflammatory property and attenuates acute lung injury in different models possibly through suppression of NF-κB activation. Biochem Pharmacol 90:73–87PubMedCrossRefGoogle Scholar