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Neurochemical Research

, Volume 40, Issue 6, pp 1095–1101 | Cite as

Capillarisin Suppresses Lipopolysaccharide-Induced Inflammatory Mediators in BV2 Microglial Cells by Suppressing TLR4-Mediated NF-κB and MAPKs Signaling Pathway

  • Zhen YuEmail author
  • Ling Tang
  • Lifen Chen
  • Jifang Li
  • Wanfu Wu
  • Changlin Hu
Original Paper

Abstract

Capillarisin, one of the major bioactive compounds derived from Artemisia capillaries Thunb, has been reported to have extensive pharmacological properties, such as ant-inflammatory and anti-nociceptive activities. However, the molecular mechanisms responsible for the anti-inflammatory activity of capillarisin have not been elucidated in microglia. In the present study, we investigated the anti-inflammatory effects and molecular mechanisms of capillarisin on LPS-stimulated BV2 microglial cells. The effects of capillarisin on inflammatory mediators TNF-α, IL-6, IL-1β, NO and PGE2 were detected. The effects of capillarisin on NF-κB and MAPK activation were detected by western blotting. The results showed that capillarisin suppressed LPS-induced TNF-α, IL-6, IL-1β, NO and PGE2 production in a dose-dependent manner. Capillarisin also inhibited LPS-induced TLR4 expression, NF-κB and MAPKs activation in BV2 microglia. In conclusion, capillarisin inhibited LPS-induced inflammation by blocking TLR4-mediated NF-κB and MAPKs activation in BV2 microglia.

Keywords

Capillarisin LPS NF-κB Microglia 

Notes

Acknowledgments

This study was supported by grants from the Natural Science Foundation of Chongqing (Grant No. CSTC2012JJA10067).

Conflict of interest

All authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zhen Yu
    • 1
    Email author
  • Ling Tang
    • 2
  • Lifen Chen
    • 1
  • Jifang Li
    • 1
  • Wanfu Wu
    • 3
  • Changlin Hu
    • 1
  1. 1.Department of Neurology, The Second Affiliated HospitalChongqing University of Medical SciencesChongqingPeople’s Republic of China
  2. 2.Department of Neurology, The Fifth Affiliated HospitalChongqing University of Medical SciencesChongqingPeople’s Republic of China
  3. 3.Department of Neurology, The First Affiliated HospitalChongqing University of Medical SciencesChongqingPeople’s Republic of China

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