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Neurochemical Research

, Volume 40, Issue 1, pp 204–215 | Cite as

The Role of Homer1b/c in Neuronal Apoptosis Following LPS-Induced Neuroinflammation

  • Zhiming Cui
  • Li Zhou
  • Chun Liu
  • Guanghui Zhu
  • Xinmin Wu
  • Yaohua Yan
  • Xiaopeng Xia
  • Zhiyun Ben
  • Yan Song
  • Ying Zhou
  • Haiyan Zhang
  • Dongmei ZhangEmail author
Original Paper

Abstract

Homer, also designated Vesl, is one member of the newly found postsynaptic density scaffold proteins, playing a vital role in maintaining synaptic integrity, regulating intracellular calcium mobilization, and being critical for the regulation of cellular apoptosis. However, its function in the inflamed central nervous system (CNS) is not fully elucidated. Here, we investigated the role of Homer1b/c, a long form of Homer1, in lipopolysaccharide (LPS) induced neuroinflammation in CNS. Western blot analysis indicated that LPS administration significantly increased the expression of Homer1b/c in rat brain. Moreover, double immunofluorescent staining suggested Homer1b/c was mainly distributed in the cytoplasm of neurons and had a close association with cleaved caspase-3 level in neurons in rat brain after LPS injection. In vitro studies indicated that up-regulation of Homer1b/c might be related to the subsequent apoptosis in neurons treated by conditioned media (CM), collected from LPS-stimulated mixed glial cultures (MGC). We also found down-regulation of Homer1b/c partly blocked the increase of cleaved caspase-3 and the proportion of Bax/Bcl-2 in neurons induced by MGC-CM. Taken together, these findings suggested that Homer1b/c might promote neuronal apoptosis via the Bax/Bcl-2 dependent pathway during neuroinflammation in CNS, and inhibiting Homer1b/c expression might provide a novel neuroprotective strategy against the inflammation-related neuronal apoptosis.

Keywords

Homer1b/c Neuroinflammation Neuronal apoptosis Lipopolysaccharide Rat 

Notes

Acknowledgments

This study was supported by the Nantong City Social Development Projects Funds (HS 2012032) and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), Nantong University graduate scientific and technological innovation projects (YKC13069).

Conflict of interest

All authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Zhiming Cui
    • 1
  • Li Zhou
    • 2
  • Chun Liu
    • 3
  • Guanghui Zhu
    • 1
  • Xinmin Wu
    • 1
  • Yaohua Yan
    • 1
  • Xiaopeng Xia
    • 1
  • Zhiyun Ben
    • 4
  • Yan Song
    • 4
  • Ying Zhou
    • 4
  • Haiyan Zhang
    • 4
  • Dongmei Zhang
    • 5
    Email author
  1. 1.Department of Orthopaedics, The Second Affiliated Hospital of Nantong UniversityJiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong UniversityNantongPeople’s Republic of China
  2. 2.Department of Pharmacology, Pharmacy CollegeNantong UniversityNantongPeople’s Republic of China
  3. 3.Laboratory animal centerNantong UniversityNantongPeople’s Republic of China
  4. 4.Department of Immunology, Medical CollegeNantong UniversityNantongPeople’s Republic of China
  5. 5.Department of Pathogen Biology, Medical CollegeNantong UniversityNantongPeople’s Republic of China

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