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Neurochemical Research

, Volume 39, Issue 7, pp 1338–1345 | Cite as

Effects of Aluminium on β-Amyloid (1–42) and Secretases (APP-Cleaving Enzymes) in Rat Brain

  • Linping Wang
  • Jiali Hu
  • Yue Zhao
  • Xiaoting Lu
  • Qinli Zhang
  • Qiao NiuEmail author
Original Paper

Abstract

Chronic administration of aluminium has been proposed as an environmental factor that may affect some pathological changes related to neurotoxicity and Alzheimer’s disease (AD). The abnormal generation and deposition of β-amyloid (Aβ) in senile plaques are hallmark features in the brains of AD patients. Furthermore, Aβ is generated by the sequential cleavage of the amyloid precursor protein (APP) via the APP cleaving enzyme (α-secretase, or β-secretase) and γ-secretase. In the present study, we investigated the modulation of Aβ deposition and neurotoxicity in aluminium-maltolate-treated (0, 15, 30, 45 mmol/kg body weight via intraperitoneal injection) in experimental rats. We measured Aβ1–40 and Aβ1–42 in the cortex and hippocampus in rat brains using ELISA. Subtypes of α-secretase, β-secretase, and γ-secretase, including ADAM9, ADAM10, ADAM17 (TACE), BACE1, presenilin 1 (PS1) and nicastrin (NCT), were determined using western blotting analyses. These results indicated that aluminium-maltolate induced an AD-like behavioural deficit in rats at 30 and 45 mmol/kg body weight. Moreover, the Aβ1–42 content increased significantly, both in the cortex and hippocampus, although no changes were observed in Aβ1–40. Furthermore, ADAM9, ADAM10, and ADAM17 decreased significantly; in contrast, BACE1, PS1, and NCT showed significant increase. Taken together, these results suggest that the changes in secretases may correlate to the abnormal deposition of Aβ by aluminium in rat brains.

Keywords

Aluminium β-Amyloid α-Secretase β-Secretase γ-Secretase 

Notes

Acknowledgments

This work was supported by the National Nature Science Foundation of China (81302410).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Linping Wang
    • 1
  • Jiali Hu
    • 1
  • Yue Zhao
    • 1
  • Xiaoting Lu
    • 1
  • Qinli Zhang
    • 1
  • Qiao Niu
    • 1
    Email author
  1. 1.School of Public HealthShanxi Medical UniversityTaiyuanChina

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