Neurochemical Research

, Volume 38, Issue 10, pp 2136–2147 | Cite as

Possible Role of Oxidative Stress and Brain Derived Neurotrophic Factor in Triazophos Induced Cognitive Impairment in Rats

  • Smita Jain
  • Basu Dev BanerjeeEmail author
  • Rafat Sultana Ahmed
  • Vinod Kumar Arora
  • Pramod Kumari Mediratta
Original Paper


Triazophos, O,O-diethyl-1-H-1,2,4-triazol-3-yl phosphorothioate, (TZ) is an organophosphate pesticide widely used as an insecticide in agriculture fields, however, its adverse effects on cognitive function remain unknown till date. The present study was designed to identify the effect of TZ on cognitive function in order to gain an insight into the molecular mechanism(s) probably involved in TZ induced toxicity. Wistar male albino rats were orally administered with TZ at 8.2 mg/kg bw daily for 30 days. Cognitive function was assessed by evaluating step down latency (SDL) in passive avoidance apparatus, transfer latency (TL) on elevated plus maze and escape latency (EL) using morris water maze. The biochemical changes, in terms of malondialdehyde (MDA), reduced glutathione (GSH) and brain derived neurotrophic factor (BDNF) levels were evaluated in hippocampi regions. Relative mRNA expression and protein expression of BDNF were also evaluated. The results demonstrated that rats treated with TZ showed significantly (p < 0.01) reduced SDL and prolonged TL and EL as compared to control group rats. Moreover, significantly low (p < 0.01) mRNA expression and protein levels (p < 0.001) of BDNF, increased MDA and reduced GSH levels were observed in TZ treated rats. The study concludes that chronic exposure to TZ significantly impairs the learning and memory which may be attributed to the significantly reduced mRNA and protein expression of BDNF in hippocampus. Moreover, BDNF is negatively correlated to MDA levels and positively correlated to GSH levels. Hence, it can be suggested that interplay between BDNF and oxidative stress plays an important role in mediating the toxic effects of TZ.


Triazophos Organophosphorus pesticide Cognitive function Brain derived neurotrophic factor Oxidative stress Memory Neurotoxicity 





Brain derived neurotrophic factor


Reduced glutathione




Step down latency


Transfer latency


Escape latency


Morris water maze



One of the authors ‘Ms Smita Jain’ is grateful to Council of Scientific and Industrial Research (CSIR) for awarding financial support as Senior Research Fellowship. The authors acknowledge Dr. Mohit Mehndiratta, Assistant Professor, Department of Biochemistry, UCMS for providing help and support during utilization of Real Time PCR system.

Conflict of interest

None declared.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Smita Jain
    • 1
  • Basu Dev Banerjee
    • 1
    Email author
  • Rafat Sultana Ahmed
    • 1
  • Vinod Kumar Arora
    • 2
  • Pramod Kumari Mediratta
    • 3
  1. 1.Environmental Biochemistry and Molecular Biology Laboratory, Department of BiochemistryUniversity College of Medical Sciences and GTB Hospital (University of Delhi)DelhiIndia
  2. 2.Histopathology Laboratory, Department of PathologyUniversity College of Medical Sciences & GTB Hospital (University of Delhi)DelhiIndia
  3. 3.Department of PharmacologyUniversity College of Medical Sciences & GTB Hospital (University of Delhi)DelhiIndia

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