Neurochemical Research

, Volume 38, Issue 3, pp 547–556

Effect of Anacyclus pyrethrum on Pentylenetetrazole-Induced Kindling, Spatial Memory, Oxidative Stress and Rho-Kinase II Expression in Mice

  • Monika Pahuja
  • Jogender Mehla
  • K. H. Reeta
  • Manjari Tripathi
  • Yogendra Kumar Gupta
Original Paper

Abstract

Anacyclus pyrethrum (A. pyrethrum) has been reported to exhibit anticonvulsant activity. In the present study, the effect of hydro-alcoholic extract of A. pyrethrum root (HEAP) on pentylenetetrazole (PTZ) induced kindling, spatial memory, oxidative stress and rho kinase (ROCK II) was assessed. Male albino mice (25–30 g) were used in the study. PTZ (35 mg/kg, i.p. on alternate days) was injected to induce kindling and PTZ (70 mg/kg, i.p) challenge was given 7 days post-kindling. HEAP was administered orally daily in the doses of 100, 250 and 500 mg/kg along with PTZ injections during the kindling process and continued till PTZ challenge post kindling. Spatial memory was assessed using Morris water maze test. Oxidative stress parameters [malondialdehyde (MDA) and reduced glutathione (GSH)] and ROCK II expression were estimated in whole brain at the end of the study. Pre-treatment with HEAP (250 and 500 mg/kg) showed significant increase in the myoclonic jerk latency and delay in the development of kindling. A significant decrease in mortality was observed at higher doses of HEAP (250 and 500 mg/kg). Pre-treatment with HEAP significantly increased the number of platform crossings and decreased the escape latency, as opposed to the PTZ group, thus showing protection against memory deficit. HEAP pre-treatment also attenuated the oxidative stress induced by PTZ kindling. PTZ induced kindling increased the ROCK II expression whereas, HEAP pre-treatment attenuated the increase in ROCK II expression. To conclude, HEAP pre-treatment showed antiepileptic effect and also showed protection against cognitive impairment by decreasing oxidative stress and ROCK II expression in PTZ kindled mice.

Keywords

Epilepsy Anacyclus pyrethrum PTZ kindling Spatial memory Oxidative stress Rho kinase II 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Monika Pahuja
    • 1
  • Jogender Mehla
    • 1
  • K. H. Reeta
    • 1
  • Manjari Tripathi
    • 2
  • Yogendra Kumar Gupta
    • 1
  1. 1.Department of PharmacologyAll India Institute of Medical SciencesNew DelhiIndia
  2. 2.Department of NeurologyAll India Institute of Medical SciencesNew DelhiIndia

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