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Neurochemical Research

, Volume 37, Issue 12, pp 2678–2685 | Cite as

Quantitative Mass Spectrometry Analysis Reveals that Deletion of the TRPV1 Receptor in Mice Alters Substance P and Neurokinin A Expression in the Central Nervous System

  • Floriane Pailleux
  • Jérôme Lemoine
  • Francis BeaudryEmail author
Original Paper

Abstract

Vanilloid receptors have a central role in the processing of nociceptive stimuli. TRPV1 null mice showed significant decrease in response to heat noxious stimuli. However, thermal sensitivity is still present suggesting that the TRPV1 is not an exclusive transducer of thermal stimuli. Additionally, tachykinin peptides play a central role in pain processing and expression levels may also contribute in modifying the pain threshold. The LC–MS/MS analysis revealed that SP and NKA were significantly down-regulated in TRPV1−/− in spinal cord and brain tissues. In spinal cord, SP concentrations were 23.4 % lower (p < 0.0049) and NKA concentrations were 22.0 % lower (p < 0.0022) in TRPV1 null mice. Additionally, brain SP concentrations were 26.9 % lower (p < 0.0260) and brain NKA concentrations were 31.9 % lower (p < 0.0063) in TRPV1 null mice. These results clearly demonstrate that TPRV1 null mice exhibit lower SP and NKA concentrations in the central nervous system. The deficit of thermal responses may also be related to the down-regulations of SP and NKA.

Keywords

TRPV1 Nociceptors Tachykinins Pain Mass spectrometry 

Abbreviations

Ac2O

Acetic anhydride

CNS

Central nervous system

FA

Formic acid

HPLC

High-performance liquid chromatography

m/z

Mass to charge ratio

MRM

Multiple reaction monitoring

MS

Mass spectrometry

NK1

Neurokinin 1 receptor

NK2

Neurokinin 2 receptor

NKA

Neurokinin A

SP

Substance P

TFA

Trifluoroacetic acid

TRPV1

Transient receptor potential vanilloid 1

WT

Wild type

Notes

Acknowledgments

This work was funded by the National Sciences and Engineering Research Council of Canada (F. Beaudry NSERC Discovery grant No. 386637-2010). F. Pailleux received a scholarship from the Coopération et mobilité Internationales Rhône-Alpes (CMIRA).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Floriane Pailleux
    • 1
    • 2
  • Jérôme Lemoine
    • 2
  • Francis Beaudry
    • 1
    Email author
  1. 1.Groupe de Recherche en Pharmacologie Animal du Québec (GREPAQ), Département de biomédecine vétérinaire, Faculté de médecine vétérinaireUniversité de MontréalSaint-HyacintheCanada
  2. 2.UMR 5280 CNRS Université de Lyon 1, Institut des Sciences AnalytiquesUniversité de LyonVilleurbanne cedexFrance

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