Neurochemical Research

, Volume 37, Issue 9, pp 1952–1958 | Cite as

Assessment of Oxidative Parameters in Rat Spinal Cord After Chronic Constriction of the Sciatic Nerve

  • Cristina S. B. Goecks
  • Andréa Horst
  • Maira S. Moraes
  • Taína Scheid
  • Carolina Kolberg
  • Adriane Belló-Klein
  • Wania A. PartataEmail author
Original Paper


Although reactive oxygen species (ROS) are involved in neuropathic pain, the direct relationship between these species and chronic constriction of sciatic nerve (CCI) has not been studied in spinal cord. Thus, this study induced CCI in rats and these animals were sacrificed 3 and 10 days after the surgical procedure to determine the superoxide dismutase (SOD) and catalase activities, as well as ascorbic acid, hydrogen peroxide (H2O2) and lipid hydroperoxide levels in lumbosacral spinal cord. Von Frey Hair and hot plate tests were performed to assess the degree of mechanical and thermal hyperalgesia at days 0, 3 and 10. The results showed that CCI significantly induced mechanical and thermal hyperalgesia at days 3 and 10. Parallel there was increase in spinal cord lipid hydroperoxide at days 3 and 10 in rats submitted to CCI. In Sham rats a significant increase in this parameter occurred at day 10. H2O2 decreased at day 10 only in CCI group. SOD activity was decreased in Sham and CCI groups at day 3, while catalase activity was increased in CCI rats at days 3 and 10. Ascorbic acid levels were reduced only in CCI rats at day 3. Although the role of such changes is unclear, many were not specific to neuropathic pain and the differences could be related to different degrees of central sensitization in Sham and CCI rats.


Neuropathic pain Catalase activity Superoxide dismutase activity Ascorbic acid Hydrogen peroxide Lipid hydroperoxide 



This work was supported by grants from Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Cristina S. B. Goecks
    • 1
  • Andréa Horst
    • 1
  • Maira S. Moraes
    • 1
  • Taína Scheid
    • 1
  • Carolina Kolberg
    • 1
  • Adriane Belló-Klein
    • 1
  • Wania A. Partata
    • 1
    Email author
  1. 1.Departamento de Fisiologia, Instituto de Ciências Básicas da SaúdeUFRGSPorto AlegreBrazil

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