Neurochemical Research

, Volume 37, Issue 2, pp 417–427

Mimosine-Induced Apoptosis in C6 Glioma Cells Requires the Release of Mitochondria-Derived Reactive Oxygen Species and p38, JNK Activation

  • Shanlou Qiao
  • Keiko Murakami
  • Qinghong Zhao
  • Baoling Wang
  • Hisao Seo
  • Hitoshi Yamashita
  • Xiaotao Li
  • Takashi Iwamoto
  • Masatoshi Ichihara
  • Masataka Yoshino
Original Paper

Abstract

Growth-inhibitory effects of mimosine, a plant amino acid, on rat C6 glioma cells were analyzed. Mimosine markedly inhibited proliferation and induced apoptosis of C6 glioma cells in a dose- and time-dependent manner. Mimosine-mediated apoptosis was accompanied by promoting reactive oxygen species (ROS) generation in mitochondria, and by decreased mitochondrial membrane potential (Δψ), and release of cytochrome c from mitochondria, followed by caspase 3 activation. Furthermore, mimosine increased the phosphorylation level of c-Jun-N-terminal protein kinase and p38, which was the downstream effect of ROS accumulation. Mimosine was confirmed to show profound effects on apoptosis of C6 glioma cells by ROS-regulated mitochondria pathway, and these results bear on the hypothesized potential for mimosine as promising agents in the treatment of malignant gliomas.

Keywords

C6 glioma cell Mimosine ROS Apoptosis Mitochondria 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Shanlou Qiao
    • 1
  • Keiko Murakami
    • 2
  • Qinghong Zhao
    • 3
  • Baoling Wang
    • 3
  • Hisao Seo
    • 1
  • Hitoshi Yamashita
    • 1
  • Xiaotao Li
    • 4
  • Takashi Iwamoto
    • 1
  • Masatoshi Ichihara
    • 1
  • Masataka Yoshino
    • 5
  1. 1.Department of Biomedical Sciences, College of Life and Health SciencesChubu UniversityAichiJapan
  2. 2.Department of BiochemistryAichi Medical University School of MedicineNagakute, AichiJapan
  3. 3.Department of SurgeryThe Second Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  4. 4.Institute of Biomedical SciencesEast China Normal UniversityShanghaiChina
  5. 5.Department of Food and Nutritional EnvironmentKinjo Gakuin UniversityMoriyama-ku, NagoyaJapan

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