Neurochemical Research

, Volume 37, Issue 2, pp 261–267 | Cite as

Effects of Cu,Zn-Superoxide Dismutase on Cell Proliferation and Neuroblast Differentiation in the Mouse Dentate Gyrus

  • Dae Young Yoo
  • Bich Na Shin
  • In Hye Kim
  • Woosuk Kim
  • Dae Won Kim
  • Ki-Yeon Yoo
  • Jung Hoon Choi
  • Choong Hyun Lee
  • Yeo Sung Yoon
  • Soo Young Choi
  • Moo-Ho Won
  • In Koo Hwang
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Oxidative stress is one of the most important factors in reducing adult hippocampal neurogenesis in the adult brain. In this study, we observed the effects of Cu,Zn-superoxide dismutase (SOD1) on lipid peroxidation, cell proliferation, and neuroblast differentiation in the mouse dentate gyrus using malondialdehyde (MDA), Ki67, and doublecortin (DCX), respectively. We constructed an expression vector, PEP-1, fused PEP-1 with SOD1, and generated PEP-1-SOD1 fusion protein. We administered PEP-1 and 100 or 500 μg PEP-1-SOD1 intraperitoneally once a day for 3 weeks and sacrificed at 30 min after the last administrations. PEP-1 administration did not change the MDA levels compared to those in the vehicle-treated group, while PEP-1-SOD1 treatment significantly reduced MDA levels compared to the vehicle-treated group. In the PEP-1-treated group, the number of Ki67-positive nuclei was similar to that in the vehicle-treated group. In the 100 μg PEP-1-SOD1-treated group, the number of Ki67-positive nuclei was slightly decreased; however, in the 500 μg PEP-1-SOD1-treated group, Ki67-positive nuclei were decreased to 78.5% of the vehicle-treated group. The number of DCX-positive neuroblasts in the PEP-1-treated group was similar to that in the vehicle-treated group. However, the arborization of DCX-positive neuroblasts was significantly decreased in both the 100 and 500 μg PEP-1-SOD1-treated groups compared to that in the vehicle-treated group. The number of DCX-positive neuroblasts with tertiary dendrites was markedly decreased in the 500 μg PEP-1-SOD1-treated group. These results suggest that a SOD1 supplement to healthy mice may not be necessary to modulate cell proliferation and neuroblast differentiation in the dentate gyrus.

Keywords

PEP-1-SOD1 fusion protein Neurogenesis Subgranular zone Oxidative stress Lipid peroxidation 
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Notes

Acknowledgments

The authors would like to thank Mr. Seung Uk Lee and Mrs. Hyun Sook Kim for their technical help in this study. This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0010580), and by a grant (2010K000823) from Brain Research Center of the twenty-first Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dae Young Yoo
    • 1
  • Bich Na Shin
    • 2
  • In Hye Kim
    • 2
  • Woosuk Kim
    • 1
  • Dae Won Kim
    • 3
  • Ki-Yeon Yoo
    • 4
  • Jung Hoon Choi
    • 5
  • Choong Hyun Lee
    • 6
  • Yeo Sung Yoon
    • 1
  • Soo Young Choi
    • 3
  • Moo-Ho Won
    • 6
  • In Koo Hwang
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea
  2. 2.Department of Physiology, College of MedicineHallym UniversityChuncheonSouth Korea
  3. 3.Department of Biomedical Sciences, and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea
  4. 4.Department of Oral Anatomy, College of DentistryKangneung-Wonju National UniversityKangneungSouth Korea
  5. 5.Department of Anatomy, College of Veterinary MedicineKangwon National UniversityChuncheonSouth Korea
  6. 6.Department of Neurobiology, School of MedicineKangwon National UniversityChuncheonSouth Korea

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