Neurochemical Research

, 36:1850 | Cite as

Synergistic Effects of Sodium Butyrate, a Histone Deacetylase Inhibitor, on Increase of Neurogenesis Induced by Pyridoxine and Increase of Neural Proliferation in the Mouse Dentate Gyrus

  • Dae Young Yoo
  • Woosuk Kim
  • Sung Min Nam
  • Dae Won Kim
  • Jin Young Chung
  • Soo Young Choi
  • Yeo Sung Yoon
  • Moo-Ho WonEmail author
  • In Koo HwangEmail author
Original Paper


We previously observed that pyridoxine (vitamin B6) significantly increased cell proliferation and neuroblast differentiation without any neuronal damage in the hippocampus. In this study, we investigated the effects of sodium butyrate, a histone deacetylase (HDAC) inhibitor which serves as an epigenetic regulator of gene expression, on pyridoxine-induced neural proliferation and neurogenesis induced by the increase of neural proliferation in the mouse dentate gyrus. Sodium butyrate (300 mg/kg, subcutaneously), pyridoxine (350 mg/kg, intraperitoneally), or combination with sodium butyrate were administered to 8-week-old mice twice a day and once a day, respectively, for 14 days. The administration of sodium butyrate significantly increased acetyl-histone H3 levels in the dentate gyrus. Sodium butyrate alone did not show the significant increase of cell proliferation in the dentate gyrus. But, pyridoxine alone significantly increased cell proliferation. Sodium butyrate in combination with pyridoxine robustly enhanced cell proliferation and neurogenesis induced by the increase of neural proliferation in the dentate gyrus, showing that sodium butyrate treatment distinctively enhanced development of neuroblast dendrites. These results indicate that an inhibition of HDAC synergistically promotes neurogenesis induced by a pyridoxine and increase of neural proliferation.


Hippocampus Neurogenesis Vitamin B6 Histone deacetylase inhibitor Ki67 Doublecortin 



The authors would like to thank Mr. Seung Uk Lee and Mrs. Hyun Sook Kim for their technical help in this study. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST), Republic of Korea (2010-0007711).


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Dae Young Yoo
    • 1
  • Woosuk Kim
    • 1
  • Sung Min Nam
    • 1
  • Dae Won Kim
    • 2
  • Jin Young Chung
    • 3
  • Soo Young Choi
    • 2
  • Yeo Sung Yoon
    • 1
  • Moo-Ho Won
    • 4
    Email author
  • In Koo Hwang
    • 1
    Email author
  1. 1.Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea
  2. 2.Department of Biomedical Sciences, and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea
  3. 3.Department of NeurologySeoul National University HospitalSeoulSouth Korea
  4. 4.Department of Neurobiology, School of MedicineKangwon National UniversityChuncheonSouth Korea

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