Neurochemical Research

, Volume 36, Issue 8, pp 1501–1511 | Cite as

Hydrogen-Rich Saline is Cerebroprotective in a Rat Model of Deep Hypothermic Circulatory Arrest

  • Li Shen
  • Jun Wang
  • Kun Liu
  • Chunzhang Wang
  • Changtian Wang
  • Haiwei Wu
  • Qiang Sun
  • Xuejun Sun
  • Hua Jing
Original Paper


Deep hypothermic circulatory arrest (DHCA) has been widely used in the operations involving the aortic arch and brain aneurysm since 1950s; but prolonged DHCA contributes significantly to neurological deficit which remains a major cause of postoperative morbidity and mortality. It has been reported that hydrogen exerts a therapeutic antioxidant activity by selectively reducing hydroxyl radical. In this study, DHCA treated rats developed a significant oxidative stress, inflammatory reaction and apoptosis. The administration of HRS resulted in a significant decrease in the brain injury, together with lower production of IL-1β, TNF-α, 8-OHdG and MDA as well as decreased activity of NOS while increased activity of SOD. The apoptotic index as well as the expressions of caspase-3 in brain tissue was significantly decreased after treatment. HRS administration significantly attenuated the severity of DHCA induced brain injury by mechanisms involving amelioration of oxidative stress, down-regulation of inflammatory factors and reduction of apoptosis.


Cerebral protection Deep hypothermic circulatory arrest Hydrogen Oxidative stress Rat 



Deep hypothermic circulatory arrest




Enzyme-linked immunosorbent assay


Electromobility Shift Analysis


Hydrogen-rich saline






Nuclear factor-κB


Nitric oxide synthase


Reactive nitrogen species


Reactive oxygen species


Systemic inflammatory response syndrome


Superoxide dismutase


Tumor necrosis factor-α



This study was supported by grant from the National Natural Science Foundation of China (No. 30972969). We sincerely thank Dr. Geng-bao Feng and Miss Kang-li Hui for their excellent technical assistance. We also sincerely thank Dr. Bing Guan for his assistance with pathology analysis and Dr. Yi Li for language editing.

Conflict of interest

All authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Li Shen
    • 1
  • Jun Wang
    • 1
  • Kun Liu
    • 1
  • Chunzhang Wang
    • 1
  • Changtian Wang
    • 1
  • Haiwei Wu
    • 1
  • Qiang Sun
    • 2
  • Xuejun Sun
    • 2
  • Hua Jing
    • 1
  1. 1.Department of Cardiothoracic Surgery, Jinling HospitalClinical Medicine School of Nanjing UniversityNanjingPeople’s Republic of China
  2. 2.Department of Diving Medicine, Faculty of Naval MedicineSecond Military Medical UniversityShanghaiPeople’s Republic of China

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