Hydrogen-Rich Saline is Cerebroprotective in a Rat Model of Deep Hypothermic Circulatory Arrest
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Deep hypothermic circulatory arrest (DHCA) has been widely used in the operations involving the aortic arch and brain aneurysm since 1950s; but prolonged DHCA contributes significantly to neurological deficit which remains a major cause of postoperative morbidity and mortality. It has been reported that hydrogen exerts a therapeutic antioxidant activity by selectively reducing hydroxyl radical. In this study, DHCA treated rats developed a significant oxidative stress, inflammatory reaction and apoptosis. The administration of HRS resulted in a significant decrease in the brain injury, together with lower production of IL-1β, TNF-α, 8-OHdG and MDA as well as decreased activity of NOS while increased activity of SOD. The apoptotic index as well as the expressions of caspase-3 in brain tissue was significantly decreased after treatment. HRS administration significantly attenuated the severity of DHCA induced brain injury by mechanisms involving amelioration of oxidative stress, down-regulation of inflammatory factors and reduction of apoptosis.
KeywordsCerebral protection Deep hypothermic circulatory arrest Hydrogen Oxidative stress Rat
Deep hypothermic circulatory arrest
Enzyme-linked immunosorbent assay
Electromobility Shift Analysis
Nitric oxide synthase
Reactive nitrogen species
Reactive oxygen species
Systemic inflammatory response syndrome
Tumor necrosis factor-α
This study was supported by grant from the National Natural Science Foundation of China (No. 30972969). We sincerely thank Dr. Geng-bao Feng and Miss Kang-li Hui for their excellent technical assistance. We also sincerely thank Dr. Bing Guan for his assistance with pathology analysis and Dr. Yi Li for language editing.
Conflict of interest
All authors declare that they have no conflict of interest.
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