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Neurochemical Research

, Volume 35, Issue 11, pp 1828–1839 | Cite as

Sulbutiamine Counteracts Trophic Factor Deprivation Induced Apoptotic Cell Death in Transformed Retinal Ganglion Cells

  • Kui Dong Kang
  • Aman Shah Abdul Majid
  • Kyung-A Kim
  • Kyungsu Kang
  • Hong Ryul Ahn
  • Chu Won Nho
  • Sang Hoon Jung
ORIGINAL PAPER

Abstract

Sulbutiamine is a highly lipid soluble synthetic analogue of vitamin B1 and is used clinically for the treatment of asthenia. The aim of our study was to demonstrate whether sulbutiamine is able to attenuate trophic factor deprivation induced cell death to transformed retinal ganglion cells (RGC-5). Cells were subjected to serum deprivation for defined periods and sulbutiamine at different concentrations was added to the cultures. Various procedures (e.g. cell viability assays, apoptosis assay, reactive oxygen species analysis, Western blot analysis, flow cytometric analysis, glutathione (GSH) and glutathione-S-transferase (GST) measurement) were used to demonstrate the effect of sulbutiamine. Sulbutiamine dose-dependently attenuated apoptotic cell death induced by serum deprivation and stimulated GSH and GST activity. Moreover, sulbutiamine decreased the expression of cleaved caspase-3 and AIF. This study demonstrates for the first time that sulbutiamine is able to attenuate trophic factor deprivation induced apoptotic cell death in neuronal cells in culture.

Keywords

Sulbutiamine Trophic factor deprivation RGC-5 cells Glaucoma 

Notes

Acknowledgments

The authors thank Alcon Research, Ltd., for providing the RGC-5 cells. This work was supported by grant No.RTI05-01-02 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kui Dong Kang
    • 1
    • 2
  • Aman Shah Abdul Majid
    • 1
    • 3
  • Kyung-A Kim
    • 4
  • Kyungsu Kang
    • 4
  • Hong Ryul Ahn
    • 4
  • Chu Won Nho
    • 4
  • Sang Hoon Jung
    • 4
  1. 1.Department of Ophthalmology, Oxford Eye HospitalUniversity of OxfordHeadington, OxfordUK
  2. 2.Department of OphthalmologyThe Catholic University of KoreaSeoulKorea
  3. 3.Advanced Medical and Dental InstituteUniversiti Sains MalaysiaPenangMalaysia
  4. 4.Natural Products Research CenterKorea Institute of Science and Technology (KIST) Gangneung InstituteDaejeon-dong, GangneungKorea

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