Neurochemical Research

, Volume 35, Issue 10, pp 1530–1537

The Role of Oxidative Stress in Amyotrophic Lateral Sclerosis and Parkinson’s Disease

  • Athan Baillet
  • Vanessa Chanteperdrix
  • Candice Trocmé
  • Pierre Casez
  • Catherine Garrel
  • Gérard Besson
ORIGINAL PAPER

Abstract

We examined oxidative stress markers of 31 patients suffering from ALS, 24 patients suffering from PD and 30 healthy subjects were included. We determined the plasma levels of lipid peroxidation (malondialdehyde, MDA), of protein oxidative lesions (plasma glutathione, carbonyls and thiols) and the activity of antioxidant enzymes i.e. erythrocyte Cu,Zn-Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px) and catalase. MDA and thiols were significantly different in both neurodegenerative diseases versus control population. A trend for an enhancement of oxidized glutathione was noted in ALS patients. Univariate analysis showed that SOD activity was significantly decreased in ALS and GSH-Px activity was decreased in PD. After adjusting for demographic parameters and enzyme cofactors, we could emphasize a compensatory increase of SOD activity in PD. Different antioxidant systems were not involved in the same way in ALS and PD, suggesting that oxidative stress may be a cause rather than a consequence of the neuronal death.

Keywords

Oxidative stress Reactive oxygen species Neurodegeneration Trace element Amyotrophic lateral sclerosis Parkinson’s disease Glutathione peroxidase Superoxide dismutase 

Abbreviations

ALS

Amyotrophic lateral sclerosis

EDTA

Ethylenediaminetetraacetic acid

GSH-Px

Glutathione peroxidase

MDA

Malondialdehyde

MOPS

3-N-morpholinopropaneslfonic acid

PD

Parkinson’s disease

SOD

Cu,Zn-Superoxide dismutase

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Athan Baillet
    • 1
    • 5
  • Vanessa Chanteperdrix
    • 2
  • Candice Trocmé
    • 3
  • Pierre Casez
    • 4
  • Catherine Garrel
    • 2
  • Gérard Besson
    • 1
  1. 1.Neurology DepartmentHôpital A. MichallonGrenoble Cedex 9France
  2. 2.Biology DepartmentHôpital A MichallonGrenoble Cedex 9France
  3. 3.Laboratory of Enzymology/DBPCCHU Hôpital MichallonGrenoble Cedex 9France
  4. 4.Medical Information UnitA. MichallonGrenoble Cedex 9France
  5. 5.Grenoble UniversityHôpital A. MichallonGrenoble Cedex 9France

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