Neurochemical Research

, Volume 35, Issue 9, pp 1422–1433

Beneficial Effects of Minocycline on Cuprizone Induced Cortical Demyelination

  • Thomas Skripuletz
  • Elvira Miller
  • Darius Moharregh-Khiabani
  • Alexander Blank
  • Refik Pul
  • Viktoria Gudi
  • Corinna Trebst
  • Martin Stangel
Original Paper

Abstract

In this study, we investigated the potential of minocycline to influence cuprizone induced demyelination in the grey and white matter. To induce demyelination C57BL/6 mice were fed with cuprizone for up to 6 weeks and were analysed at different timepoints (week 0, 4, 5, 6). Mice treated with minocycline had less demyelination of the cortex and corpus callosum compared with sham treated animals. In the cortex decreased numbers of activated and proliferating microglia were found after 6 weeks of cuprizone feeding, while there were no significant effects for microglial infiltration of the corpus callosum. In addition to the beneficial effects on demyelination, minocycline prevented from motor coordination disturbance as shown in the beam walking test. For astrogliosis and the numbers of OPC and oligodendrocytes no treatment effects were found. In summary, minocycline treatment diminished the course of demyelination in the grey and white matter and prevented disturbances in motor coordination.

Keywords

Myelin Remyelination Animal model C57BL/6 mice 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Thomas Skripuletz
    • 1
  • Elvira Miller
    • 1
  • Darius Moharregh-Khiabani
    • 1
  • Alexander Blank
    • 1
  • Refik Pul
    • 1
  • Viktoria Gudi
    • 1
    • 2
  • Corinna Trebst
    • 1
  • Martin Stangel
    • 1
    • 2
  1. 1.Department of NeurologyHannover Medical SchoolHanoverGermany
  2. 2.Center for Systems NeuroscienceHannoverGermany

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