Neurochemical Research

, Volume 35, Issue 5, pp 811–819 | Cite as

The Fat-1 Mouse has Brain Docosahexaenoic Acid Levels Achievable Through Fish Oil Feeding

  • Sarah K. Orr
  • Jasmin Y. M. Tong
  • Jing X. Kang
  • David W. L. MaEmail author
  • Richard P. Bazinet
Original Paper


Fat-1 transgenic mice endogenously convert n-6 to n-3 polyunsaturated fatty acids (PUFA). The aims of this study were to test whether a) fish oil feeding can attain similar brain n-3 PUFA levels as the fat-1 mouse, and b) fat-1 mouse brain docosahexaenoic acid (22:6n-3; DHA) levels can be potentiated by fish oil feeding. Fat-1 mice and their wildtype littermates consumed either a 10% safflower oil (SO) or a 2% fish oil and 8% safflower oil chow (FO). Brain total lipid and phospholipid fraction fatty acids were analyzed using GC-FID. Wildtype mice fed FO chow had similar brain levels of DHA as fat-1 mice fed SO chow. Fat-1 mice fed FO chow had similar brain n-3 PUFA levels as fat-1 mice fed SO chow. In conclusion, brain levels of DHA in the fat-1 mouse can be obtained by and were not further augmented with fish oil feeding.


Fat-1 Mouse Docosahexaenoic acid Brain n-3 PUFA Fish oil 



Arachidonic acid


Choline glycerophospholipids


Docosahexaenoic acid


Ethanolamine glycerophospholipids


2% fish oil, 8% safflower oil chow


n-9 oleic acid


Palmitic acid






Polyunsaturated fatty acid


10% safflower oil chow


Stearic acid



This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) to DWL Ma and RP Bazinet, and from the Canadian Institutes of Health Research to RP Bazinet. SK Orr received financial support from NSERC.

Supplementary material

11064_2010_139_MOESM1_ESM.docx (19 kb)
Supplementary material 1 (DOCX 19 kb)


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sarah K. Orr
    • 1
  • Jasmin Y. M. Tong
    • 1
  • Jing X. Kang
    • 2
  • David W. L. Ma
    • 1
    • 3
    Email author
  • Richard P. Bazinet
    • 1
  1. 1.Department of Nutritional Sciences, Faculty of MedicineUniversity of TorontoTorontoCanada
  2. 2.Department of MedicineHarvard Medical SchoolBostonUSA
  3. 3.Department of Human Health and Nutritional Sciences, College of Biological ScienceUniversity of GuelphGuelphCanada

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