The Fat-1 Mouse has Brain Docosahexaenoic Acid Levels Achievable Through Fish Oil Feeding
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Fat-1 transgenic mice endogenously convert n-6 to n-3 polyunsaturated fatty acids (PUFA). The aims of this study were to test whether a) fish oil feeding can attain similar brain n-3 PUFA levels as the fat-1 mouse, and b) fat-1 mouse brain docosahexaenoic acid (22:6n-3; DHA) levels can be potentiated by fish oil feeding. Fat-1 mice and their wildtype littermates consumed either a 10% safflower oil (SO) or a 2% fish oil and 8% safflower oil chow (FO). Brain total lipid and phospholipid fraction fatty acids were analyzed using GC-FID. Wildtype mice fed FO chow had similar brain levels of DHA as fat-1 mice fed SO chow. Fat-1 mice fed FO chow had similar brain n-3 PUFA levels as fat-1 mice fed SO chow. In conclusion, brain levels of DHA in the fat-1 mouse can be obtained by and were not further augmented with fish oil feeding.
KeywordsFat-1 Mouse Docosahexaenoic acid Brain n-3 PUFA Fish oil
2% fish oil, 8% safflower oil chow
n-9 oleic acid
Polyunsaturated fatty acid
10% safflower oil chow
This work was supported by grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) to DWL Ma and RP Bazinet, and from the Canadian Institutes of Health Research to RP Bazinet. SK Orr received financial support from NSERC.
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