Neurochemical Research

, Volume 35, Issue 5, pp 789–796 | Cite as

Leucine-Rich Glioma Inactivated 3 Induces Neurite Outgrowth Through Akt and Focal Adhesion Kinase

  • Woo-Jae Park
  • Yun Young Lim
  • Nyoun Soo Kwon
  • Kwang Jin Baek
  • Dong-Seok Kim
  • Hye-Young YunEmail author
Original Paper


Leucine-rich glioma inactivated 3 (LGI3) is a secreted protein that belongs to LGI/epitempin family. LGI3 is highly expressed in brain in a transcriptionally and developmentally regulated manner. Here we found that LGI3 induced neurite outgrowth in Neuro-2a cells and dorsal root ganglia explants. LGI3 treatment or overexpression increased neurite outgrowth and knockdown of LGI3 by siRNA had opposite effect. LGI3 treatment increased phosphorylation of Akt and a 125-kDa protein. Immunoprecipitation identified the 125-kDa protein as focal adhesion kinase (FAK). LGI3 overexpression increased phospho-Akt, phospho-FAK and FAK protein. Inhibition of Akt activation by PI3 kinase inhibitor attenuated LGI3-induced FAK phosphorylation and neurite outgrowth. Taken together, we propose that LGI3 is a neuritogenic factor whose signaling pathway involves Akt-mediated FAK activation.


LGI3 Neurite Akt FAK Phosphorylation Differentiation 



We thank Dr. J. L. Noebels (Baylor College of Medicine) for providing pcDNA3.1-LGI3-myc. This research was supported by the Chung-Ang University Research Grants in 2010.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Woo-Jae Park
    • 1
  • Yun Young Lim
    • 2
  • Nyoun Soo Kwon
    • 1
  • Kwang Jin Baek
    • 1
  • Dong-Seok Kim
    • 1
  • Hye-Young Yun
    • 1
    Email author
  1. 1.Department of BiochemistryChung-Ang University College of MedicineSeoulRepublic of Korea
  2. 2.Department of DermatologyChung-Ang University College of MedicineSeoulRepublic of Korea

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