Neurochemical Research

, Volume 34, Issue 12, pp 2089–2101

Effect of Intranigral Injection of GDNF and EGF on the Survival and Possible Differentiation Fate of Progenitors and Immature Neurons in 6-OHDA-Lesioned Rats

  • Zheng-Quan Yu
  • Ji-Hui Zha
  • Hong-Mei Liu
  • Yan-Xia Ding
  • Yan-Qian Wang
  • Hong-Jun Wang
  • Dian-Shuai Gao
Original Paper
  • 106 Downloads

Abstract

We investigated the survival and the possible differentiation fate of the progenitors and immature neurons in the pars compacta of the substantia nigra (SNc) by intranigral injection of a glial cell line-derived neurotropic factor (GDNF) or glial cell line-derived neurotropic factor plus epidermal growth factor (EGF + GDNF) in 6-hydroxydopamine (6-OHDA)-lesioned rats. First, we performed behavioral tests by postural asymmetry and forelimb akinesia on the rats injected with 6-OHDA in striatum at day 7, and selected the qualified model according to the results. Then, intranigral GDNF or EGF + GDNF treatment was administered in the qualified PD model rats. On day 21, behavioral tests were performed with these rats; and then the rats were sacrificed for analyses of β-tubulin isotype-III (Tuj1), nestin, glial fibrillary acidic protein (GFAP), and tyrosine hydroxylase (TH) by immunohistochemistry and Western blotting. The results indicated that GDNF could promote the survival of the progenitor cells and immature neurons in rat SNc following 6-OHDA lesion. Moreover, EGF is capable of enhancing the survival effect of GDNF on the progenitor cells and immature neurons in SNc. On day 21, rapid functional recovery from the lesion-induced behavioral asymmetries was observed in the GDNF or EGF + GDNF-treated rats, and the numbers of TH-positive neurons increased in SNc, suggesting that the rats might generate new dopaminergic neurons. Thus, our study provides the new insight that the progenitors and immature neurons in SNc of 6-OHDA-lesioned rats might be able to differentiate toward the dopaminergic neurons fate subsequent to treatment with GDNF or EGF + GDNF.

Keywords

6-OHDA GDNF and EGF Parkinson’s disease Progenitor cells Immature neurons Substantia nigra pars compacta 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Zheng-Quan Yu
    • 1
  • Ji-Hui Zha
    • 1
  • Hong-Mei Liu
    • 2
  • Yan-Xia Ding
    • 2
  • Yan-Qian Wang
    • 2
  • Hong-Jun Wang
    • 2
  • Dian-Shuai Gao
    • 2
  1. 1.Department of NeurosurgeryXuzhou Medical CollegeXuzhouPeople’s Republic of China
  2. 2.Department of NeurobiologyXuzhou Medical CollegeXuzhouPeople’s Republic of China

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