Previous studies demonstrated the efficacy of electro-acupuncture (EA) in relieving chronic visceral hypersensitivity (CVH) in IBS rats. However, ST25 which is a key acupoint for patients with IBS has not been reported in these experiments. Eight CVH rats were treated by EA at both ST25 and ST37 for 20 min, once daily for seven consecutive days, model rats (n = 8) and normal rats (n = 8) as controls. After the first EA treatment, the abdominal withdrawal reflex scores were investigated to evaluate the pain threshold. After seven EA treatments, the concentrations of 5-hydroxytryptamine (5-HT), 5-HT3 receptor (5-HT3R) and 5-HT4 receptor (5-HT4R) in colon tissue were assayed quantitatively by enzyme-linked immunosorbent assay. The results showed that EA improved the pain threshold of CVH rats, reduced the 5-HT concentration and increased the 5-HT4R concentration, but had no effect on the 5-HT3R concentration. Further studies are needed to optimize the choice of two-matching points for EA in the treatment of CVH rats.
Electro-acupuncture Chronic visceral hypersensitivity Irritable bowel syndrome 5-Hydroxytryptamine Rat
Saito YA, Schoenfeld P, Locke GR 3rd (2002) The epidemiology of irritable bowel syndrome in North America: a systematic review. Am J Gastroenterol 97:1910–1915PubMedGoogle Scholar
Forbes A, Jackson S, Walter C et al (2005) Acupuncture for irritable bowel syndrome:a blinded placebo-controlled trial. World J Gastroenterol 11:4040–4044PubMedGoogle Scholar
Crowell MD, Shetzline MA, Moses PL et al (2004) Enterochromaffin cells and 5-HT signaling in the pathophysiology of disorders of gastrointestinal function. Curr Opin Investig Drugs 5:55–60PubMedGoogle Scholar
Jin JG, Foxx-Orenstein AE, Grider JR (1999) Propulsion in guinea pig colon induced by 5-hydroxytryptamine (HT) via 5-HT4 and 5-HT3 receptors. J Pharmacol Exp Ther 288:93–97PubMedGoogle Scholar
Kozlowski CM, Green A, Grundy D et al (2000) The 5-HT(3) receptor antagonist alosetron inhibits the colorectal distention induced depressor response and spinal c-fos expression in the anaesthetised rat. Gut 46:474–480. doi:10.1136/gut.46.4.474PubMedCrossRefGoogle Scholar
Coelho A-M, Rovira P, Fioramonti J et al (2000) Antinociceptive properties of HTF 919 (tegaserod) a 5-HT4 receptor partial agonist on colorectal distension in rats. Gastroenterology 218:A835CrossRefGoogle Scholar