Neurochemical Research

, Volume 34, Issue 9, pp 1626–1634

Neuroprotective Effect of Baicalin in a Rat Model of Permanent Focal Cerebral Ischemia

  • Xian-kun Tu
  • Wei-zhong Yang
  • Song-sheng Shi
  • Chun-hua Wang
  • Chun-mei Chen
Original Paper

DOI: 10.1007/s11064-009-9953-4

Cite this article as:
Tu, X., Yang, W., Shi, S. et al. Neurochem Res (2009) 34: 1626. doi:10.1007/s11064-009-9953-4

Abstract

This investigation was performed to determine the neuroprotective effect of baicalin on permanent cerebral ischemia injury in rats and the potential mechanisms in this process. Adult male Sprague-Dawley rats underwent permanent middle cerebral artery occlusion (pMCAO). The rats were then received intraperitoneal injection with baicalin (10, 30 and 100 mg/kg) or vehicle. Morphological characteristic, neurological deficit scores, cerebral infarct volume and the enzymatic activity of myeloperoxidase (MPO) were measured 24 h after pMCAO. The mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were determined by RT-PCR. Neuronal apoptosis was determined by TUNEL staining and Western blot. Baicalin (30 and 100 mg/kg) reduced neurological deficit scores and cerebral infarct volume 24 h after pMCAO. Baicalin significantly decreased the enzymatic activity of MPO and the expression of iNOS mRNA and COX-2 mRNA in rat brain, it also significantly inhibited neuronal apoptosis and the expression of cleaved caspase-3 protein after pMCAO. Our results suggested that baicalin possesses potent anti-inflammatory and anti-apoptotic properties and attenuates cerebral ischemia injury. This protection might be associated with the downregulated expression of iNOS mRNA, COX-2 mRNA, and cleaved caspase-3 protein.

Keywords

Apoptosis Baicalin Cerebral ischemia Inflammation Neuroprotection Stroke 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xian-kun Tu
    • 1
  • Wei-zhong Yang
    • 1
  • Song-sheng Shi
    • 1
  • Chun-hua Wang
    • 1
  • Chun-mei Chen
    • 1
  1. 1.Department of NeurosurgeryThe Affiliated Union Hospital of Fujian Medical UniversityFuzhouChina

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