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Neurochemical Research

, 34:1491 | Cite as

Curcumin-Induced DNA Damage and Inhibited DNA Repair Genes Expressions in Mouse–Rat Hybrid Retina Ganglion Cells (N18)

  • Hsu-Feng Lu
  • Jai-Sing Yang
  • Kuang-Chi Lai
  • Shu-Chun Hsu
  • Shu-Ching Hsueh
  • Yuan-Liang Chen
  • Jo-Hua Chiang
  • Chi-Cheng Lu
  • Chyi Lo
  • Mei-Due Yang
  • Jing-Gung ChungEmail author
Original Paper

Abstract

Curcumin is reported to be a potent inhibitor of the initiation and promotion of many cancer cells. We investigated to examine whether or not curcumin induce DNA damage in mouse–rat hybrid retina ganglion cell line N18 cells. The Comet assay showed that incubation of N18 cells with 10, 25 and 30 μM of curcumin led to a longer DNA migration smear (Comet tail). The DNA gel electrophoresis showed that 20 μM of curcumin for 24 and 48 h treatment induced DNA damage and fragments in N18 cells. The real time PCR analysis showed that 20 μM of curcumin for 48 h treatment decreased ATM, ATR, BRCA1, 14-3-3σ, DNA-PK and MGMT mRNA, and ATM and MGMT mRNA expression were inhibited in a time-dependent manner. Our results indicate that curcumin caused DNA damage and inhibited DNA repair genes which may be the factors for curcumin-inhibited cell growth.

Keywords

Curcumin DNA damage DNA repair N18 mouse–rat hybrid retina ganglion cells 

Notes

Acknowledgments

This work was supported by Grant NSC 95-2745-B-039-002-URD from the National Science Council of Taiwan.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hsu-Feng Lu
    • 1
  • Jai-Sing Yang
    • 2
  • Kuang-Chi Lai
    • 3
  • Shu-Chun Hsu
    • 4
  • Shu-Ching Hsueh
    • 1
  • Yuan-Liang Chen
    • 5
  • Jo-Hua Chiang
    • 6
  • Chi-Cheng Lu
    • 6
  • Chyi Lo
    • 7
  • Mei-Due Yang
    • 8
  • Jing-Gung Chung
    • 9
    • 10
    Email author
  1. 1.Department of Clinical PathologyCheng Hsin Rehabilitation Medical CenterTaipeiTaiwan, ROC
  2. 2.Department of PharmacologyChina Medical UniversityTaichungTaiwan, ROC
  3. 3.Department of SurgeryChina Medical University Beigang HospitalYunlinTaiwan, ROC
  4. 4.Institute of Chinese Pharmaceutical SciencesChina Medical UniversityTaichungTaiwan, ROC
  5. 5.Department of Medical TechnologyYuan-Pei Institute of Science and TechnologyHsinchuTaiwan, ROC
  6. 6.Department of Life SciencesNational Chung Hsing UniversityTaichungTaiwan, ROC
  7. 7.School of NursingGraduate Institute of Chinese Medical Science, China Medical UniversityTaichungTaiwan, ROC
  8. 8.Department of SurgeryChina Medical University HospitalTaichungTaiwan, ROC
  9. 9.Department of Biological Science and TechnologyChina Medical UniversityTaichungTaiwan, ROC
  10. 10.Department of BiotechnologyAsia UniversityWufeng, TaichungTaiwan, ROC

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