Neurochemical Research

, Volume 35, Issue 4, pp 572–579 | Cite as

Comparison of the Efficiencies of Three Neural Induction Protocols in Human Adipose Stromal Cells

Original Paper


The aim of this study was to compare the neural differentiation potential and the expression of neurotrophic factors (NTFs) in differentiated adipose-derived stem cells (ADSCs) using three established induction protocols, serum free (Protocol 1), chemical reagents (Protocol 2), and spontaneous (Protocol 3) protocols. Protocol 1 produced the highest percentage of mature neural-like cells (MAP2ab+). Protocol 2 showed the highest percentage of immature neural-like cells (β-tubulin III+), but the neural-like state was transient and reversible. Protocol 3 caused ADSCs to differentiate spontaneously into immature neural-like cells, but not into mature neural cell types. The neural-like cells produced by Protocol 1 lived the longest in culture with little cell death, but Protocol 2 and 3 led to the significant cell death. Therefore, Protocol 1 is the most efficient among these protocols. Additionally, soon after differentiation, the mRNA levels of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in dADSCs were sharply decreased by Protocol 1 and 2 (acute induction protocol), but not by Protocol 3 (chronic induction protocol). The results indicate that NTFs played an important role in neural differentiation via acute responses to NGF and BDNF, but not chronically during the transdifferentiation process.


Mesenchymal stem cells Transdifferentiation Neural differentiation Nerve growth factors 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Neurosurgery Institute of Guangdong, Key Laboratory on Brain Function Repair and Regeneration of Guangdong, Department of Neurosurgery, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Neurosurgery, The Third Affiliated HospitalGuangzhou Medical CollegeGuangzhouChina
  3. 3.Department of NeurosurgeryThe General Hospital of Beijing PLABeijingChina
  4. 4.Department of NeurosurgeryGuangdong Provincial Hospital of Traditional Chinese MedicineGuangzhouChina

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