Neurochemical Research

, Volume 35, Issue 3, pp 357–365 | Cite as

Effect of Purple Sweet Potato Anthocyanins on β-Amyloid-Mediated PC-12 Cells Death by Inhibition of Oxidative Stress

  • Junli Ye
  • Xiangjun Meng
  • Chunling Yan
  • Chunbo Wang
Original Paper


Amyloid-beta peptide (Aβ) is known to induce the redox imbalance, mitochondrial dysfunction and caspase activation, resulting in neuronal cell death. Treatment with antioxidants provided a new therapeutic strategy for Alzheimer’s disease (AD) patients. Here we investigate the effects of purple sweet potato anthocyanins (PSPA), the known strong free radical scavengers, on Aβ toxicity in PC12 cells. The results showed that pretreatment of PC12 cells with PSPA reduced Aβ-induced toxicity, intracellular reactive oxygen species (ROS) generation and lipid peroxidation dose-dependently. In parallel, cell apoptosis triggered by Aβ characterized with the DNA fragmentation and caspase-3 activity were also inhibited by PSPA. The concentration of intracellular Ca2+ and membrane potential loss associated with cell apoptosis were attenuated by PSPA. These results suggested that PSPA could protect the PC-12 cell from Aβ-induced injury through the inhibition of oxidative damage, intracellular calcium influx, mitochondria dysfunction and ultimately inhibition of cell apoptosis. The present study indicates that PSPA may be a promising approach for the treatment of AD and other oxidative-stress-related neurodegenerative diseases.


PSPA Amyloid β peptide (Aβ) Oxidative stress Reactive oxygen species (ROS) Apoptosis PC12 cells 



Purple sweet potato anthocyanins



Alzheimer’s disease


Pheochromocytoma cells




Dichlorofluorescin diacetate


Malonyl dialdehyde


3(4,5-dimethylthiazol-2yl)2,5-diphenyl-2H-tetrazolium bromide


Optical density


Phosphate-buffered saline


Reactive oxygen species


Lipid peroxidation


Relative fluorescence unit


Changes in intracellular free calcium levels



This work was supported by a grant from the Science and Technology Bureau of Qingdao (No. 03-2-JZ-03) and the Science Foundation of Shandong Provincial Educational Department, China (Grant No. J04E17).


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Junli Ye
    • 1
  • Xiangjun Meng
    • 2
  • Chunling Yan
    • 1
  • Chunbo Wang
    • 3
  1. 1.Department of PathophysiologyMedical College, Qingdao UniversityQingdaoChina
  2. 2.Department of Psychological ConsultationNo. 7 People’s Hospital of QingdaoQingdaoChina
  3. 3.Department of PharmacologyMedical College, Qingdao UniversityQingdaoChina

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