Neurochemical Research

, 35:114 | Cite as

Expression of Adenomatous Polyposis Coli Protein in Reactive Astrocytes in Hippocampus of Kainic Acid-Induced Rat

  • Ha Na Lee
  • Gye Sun Jeon
  • Dong Woon Kim
  • Ik-Hyun Cho
  • Sa Sun Cho
Orignal Paper

Abstract

The adenomatous polyposis coli gene (APC) was initially identified through its link to colon cancer. It is associated with the regulation of cell cycle progression, survival, and differentiation of normal tissues. Recent studies have demonstrated that APC is also expressed in the adult brain at high levels. However, its role in glial cells under pathological progression remains unclear. In this study, we evaluated the expression of APC and its association with β-catenin signaling pathway, following the induction of an excitotoxic lesion by kainic acid (KA) injection, which cause pyramidal cell degeneration. APC was predominantly present in oligodendrocytes in the normal brain, but was specifically associated with activated astrocytes in the KA-treated brain. Our quantitative analysis revealed that APC significantly increased from 1 day post lesion (PI), reached peak values at 3 days PI, and decreased thereafter. The phospho-GSK3β levels also showed similar spatiotemporal patterns while β-catenin expression was reduced at 1 and then increasingly returned to normal levels at 3, 7 days PI. For the first time, our data demonstrate the injury-induced astrocytic changes in the levels of APC, GSK3β, and β-catenin in vivo, which may actively be participate in cell adhesion and in the signaling pathway regulating cell survivals during brain insults.

Keywords

Adenomatous polyposis coli Astrocytes Oligodendrocytes GSK-3beta Beta-catenin Kainic acid 

Notes

Acknowledgments

This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) [KRF-2008-331-E00005 (I00667)].

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ha Na Lee
    • 1
  • Gye Sun Jeon
    • 1
  • Dong Woon Kim
    • 2
  • Ik-Hyun Cho
    • 3
  • Sa Sun Cho
    • 1
    • 3
  1. 1.Department of AnatomySeoul National University College of MedicineSeoulSouth Korea
  2. 2.Department of AnatomyChungnam National University School of MedicineDaejeonSouth Korea
  3. 3.Department of AnatomyJeju National University School of MedicineJejuSouth Korea

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