Neurochemical Research

, Volume 34, Issue 12, pp 2251–2260

Neurite Outgrowth Effect of 4-O-methylhonokiol by Induction of Neurotrophic Factors Through ERK Activation

  • Yong Kyoung Lee
  • Im Seop Choi
  • Young Heui Kim
  • Ki Ho Kim
  • Sang Yun Nam
  • Young Won Yun
  • Moon Soon Lee
  • Ki Wan Oh
  • Jin Tae Hong
Original Paper

DOI: 10.1007/s11064-009-0024-7

Cite this article as:
Lee, Y.K., Choi, I.S., Kim, Y.H. et al. Neurochem Res (2009) 34: 2251. doi:10.1007/s11064-009-0024-7

Abstract

Compounds isolated from Magnolia officinalis such as magnolol, honokiol and obovatol exhibit several pharmacological effects on CNS including depressant, anxiolytic and anticonvulsant effects, as well as neuroprotective effects against chemical and heat damages. Recently, honokiol was found to have a neurotrophic effect in fetal rat cortical neurons. In the present study, we show that 4-O-methylhonokiol, a novel compound from Magnolia officinalis, promotes neurite outgrowth in a concentration-dependent manner in rat embryonic neuronal cells. In parallel with the neurite outgrowth activity, the expression of neurite outgrowth marker proteins is also increased by treatment with 4-O-methylhonokiol. We also found that 4-O-methylhonokiol promotes the release of NGF and BDNF into cell culture medium. In addition, lower concentration of 4-O-methylhonokiol (1 and 2 μM) further enhanced neurite outgrowth and expression of neurite outgrowth marker proteins in the presence of NGF (50 ng/ml) or BDNF (10 ng/ml). Subsequently, we found that 4-O-methylhonokiol activates ERK in a concentration-dependent manner. However, the neurite outgrowth activity and the NGF and BDNF release induced by 4-O-methylhonokiol are suppressed by an ERK-specific inhibitor. These results suggest that 4-O-methylhonokiol has the ability to induce neurite outgrowth via the increase of neurotrophic factor levels through ERK activation.

Keywords

4-O-methylhonokiol Neurite promoting effect Brain-derived neurotrophic factor Nerve growth factor Extracellular signal-regulated kinases 

Abbreviations

AD

Alzheimer’s disease

BDNF

Brain-derived neurotrophic factor

CNS

Central nervous system

ERK

Extracellular signal-regulated kinases

MAPK

Mitogen-activated protein kinase

NGF

Nerve growth factor

SDS–PAGE

Sodium dodecyl sulphate–polyacrylamide gel electrophoresis

TBI

Traumatic brain injury

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Yong Kyoung Lee
    • 1
  • Im Seop Choi
    • 1
    • 2
  • Young Heui Kim
    • 5
  • Ki Ho Kim
    • 5
  • Sang Yun Nam
    • 3
  • Young Won Yun
    • 3
  • Moon Soon Lee
    • 4
  • Ki Wan Oh
    • 1
  • Jin Tae Hong
    • 1
    • 2
  1. 1.College of PharmacyChungbuk National UniversityCheoungjuRepublic of Korea
  2. 2.Medical Research CenterChungbuk National UniversityCheoungjuRepublic of Korea
  3. 3.College of Veterinary MedicineChungbuk National UniversityCheoungjuRepublic of Korea
  4. 4.College of Agriculture, Life and EnvironmentChungbuk National UniversityCheoungjuRepublic of Korea
  5. 5.Bioland R&D CenterCheonanRepublic of Korea

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