Neurochemical Research

, 34:775

Neuroprotective Effects of Ethyl Pyruvate on Brain Energy Metabolism after Ischemia-Reperfusion Injury: A 31P-Nuclear Magnetic Resonance Study

  • Osamu Tokumaru
  • Chihiro Kuroki
  • Noriko Yoshimura
  • Tetsuro Sakamoto
  • Hidehiro Takei
  • Kazue Ogata
  • Takaaki Kitano
  • Naoko Nisimaru
  • Isao Yokoi
Original Paper

DOI: 10.1007/s11064-008-9871-x

Cite this article as:
Tokumaru, O., Kuroki, C., Yoshimura, N. et al. Neurochem Res (2009) 34: 775. doi:10.1007/s11064-008-9871-x

Abstract

The neuroprotective effects of ethyl pyruvate (EP), a stable derivative of pyruvate, on energy metabolism of rat brain exposed to ischemia-reperfusion stress were investigated by 31P-nuclear magnetic resonance (31P-NMR) spectroscopy. Recovery level of phosphocreatine after ischemia was significantly greater when superfused with artificial cerebrospinal fluid (ACSF) with 2 mM EP than when superfused with ACSF without EP. EP was neuroprotective against ischemia only when administered before the ischemic exposure. Intracellular pH during ischemia was less acidic when superfused ahead of time with EP. EP did not show neuroprotective effects in neuron-rich slices pretreated with 100 μM fluorocitrate, a selective glial poison. It was suggested that both the administration of EP before ischemic exposure and the presence of astrocytes are required for EP to exert neuroprotective effects. We suggest the potential involvement of multiple mechanisms of action, such as less acidic intracellular pH, glial production of lactate, and radical scavenging ability.

Keywords

Ethyl pyruvate Phosphocreatine ATP Brain energy metabolism 31P-NMR Ischemia-reperfusion injury 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Osamu Tokumaru
    • 1
  • Chihiro Kuroki
    • 1
  • Noriko Yoshimura
    • 1
  • Tetsuro Sakamoto
    • 1
  • Hidehiro Takei
    • 2
  • Kazue Ogata
    • 1
  • Takaaki Kitano
    • 3
  • Naoko Nisimaru
    • 1
  • Isao Yokoi
    • 1
  1. 1.Department of PhysiologyOita University Faculty of MedicineYufuJapan
  2. 2.Department of PathologyThe Methodist HospitalHoustonUSA
  3. 3.Medical Education CenterOita University Faculty of MedicineYufuJapan

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