Neurochemical Research

, Volume 34, Issue 2, pp 215–220 | Cite as

Cytoprotective Effect of Valeriana officinalis Extract on an In Vitro Experimental Model of Parkinson Disease

  • Diêgo Madureira de Oliveria
  • George Barreto
  • Deyse Valverde G. De Andrade
  • Ezequiel Saraceno
  • Laura Aon-Bertolino
  • Francisco Capani
  • Ramon Dos Santos El Bachá
  • Lisandro Diego Giraldez
Original Paper

Abstract

Parkinson′s disease (PD) is one of the most important neurodegenerative worldwide disorders. The potential cytoprotective effects of aqueous extract of Valeriana officinalis on rotenone-induced apoptosis in human neuroblastoma SH-SY5Y cells were demonstrated. The cytotoxicity, cell viability and analysis of cellular morphology were performed by MTT-tetrazole (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and phase contrast microscopy, respectively. Significant changes in the cellular morphology, and condensation of the cell body could be observed when cells were treated with 300 nM rotenone for 48 h. Three different concentrations of Valeriana officinalis extract were used (0.049, 0.098 and 0.195 mg/mL). These extracts brought about an increase of 7.0 ± 1.3%, 14.5 ± 1.3% and 14.5 ± 3.2% in cell viability. Our results indicated that neuroprotector action of the Valeriana officinalis extract provides support for later studies as they help understanding this drug for the development of cytoprotective various therapies in PD.

Keywords

Parkinson Disease SH-SY5Y cells Valeriana officinalis Cytoprotective Rotenone 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Diêgo Madureira de Oliveria
    • 1
  • George Barreto
    • 1
  • Deyse Valverde G. De Andrade
    • 1
  • Ezequiel Saraceno
    • 3
  • Laura Aon-Bertolino
    • 3
  • Francisco Capani
    • 3
  • Ramon Dos Santos El Bachá
    • 1
  • Lisandro Diego Giraldez
    • 1
    • 2
  1. 1.Laboratório de Neuroquímica e Biologia CelularUniversidade Federal da Bahia (UFBA)SalvadorBrazil
  2. 2.SalvadorBrazil
  3. 3.Departamento de Bioquímica HumanaFacultad de Medicina, Universidad de Buenos Aires (UBA)Buenos AiresArgentina

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