Neurochemical Research

, Volume 33, Issue 11, pp 2358–2363

Proteome Analysis of Cerebrospinal Fluid in Amyotrophic Lateral Sclerosis (ALS)

  • Johannes Brettschneider
  • Helga Mogel
  • Vera Lehmensiek
  • Tino Ahlert
  • Sigurd Süssmuth
  • Albert C. Ludolph
  • Hayrettin Tumani
Original Paper

DOI: 10.1007/s11064-008-9742-5

Cite this article as:
Brettschneider, J., Mogel, H., Lehmensiek, V. et al. Neurochem Res (2008) 33: 2358. doi:10.1007/s11064-008-9742-5

Abstract

Cerebrospinal fluid (CSF) is a promising source of biomarkers in amyotrophic lateral sclerosis (ALS). Using the two-dimensional difference in gel electrophoresis (2-D-DIGE), we compared CSF samples from patients with ALS (n = 14) with those from normal controls (n = 14). Protein spots that showed significant differences between patients and controls were selected for further analysis by MALDI-TOF mass spectrometry. For validation of identified spots western blot analysis and ELISA was performed. We identified 2 proteins that were upregulated and 3 proteins that were down-regulated in CSF in ALS. Of these, two proteins (Zn-alpha-2-glycoprotein and ceruloplasmin precursor protein) have not been reported in CSF of patients with ALS so far. In contrast, several other proteins (transferrin, alpha-1-antitrypsin precursor and beta-2-microglobulin) seem to be unspecifically affected in different neurological diseases and may therefore be of limited value as disease-related biochemical markers in ALS. Further evaluation of the candidate proteins identified here is necessary.

Keywords

Amyotrophic lateral sclerosis ALS Motor neuron disease MND Cerebrospinal fluid Proteome Difference in gel electrophoresis 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Johannes Brettschneider
    • 1
  • Helga Mogel
    • 1
  • Vera Lehmensiek
    • 1
  • Tino Ahlert
    • 1
  • Sigurd Süssmuth
    • 1
  • Albert C. Ludolph
    • 1
  • Hayrettin Tumani
    • 1
  1. 1.Department of NeurologyUniversity of UlmUlmGermany

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