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Neurochemical Research

, Volume 33, Issue 11, pp 2257–2262 | Cite as

DARPP-32 Expression in Rat Brain After an Inhibitory Avoidance Task

  • Daniela V. F. Rosa
  • Renan P. Souza
  • Bruno R. Souza
  • Melissa M. Guimarães
  • Daniel S. Carneiro
  • Samira S. Valvassori
  • Marcus V. Gomez
  • João Quevedo
  • Marco A. Romano-Silva
Original Paper

Abstract

Step-down inhibitory avoidance (IA) is usually acquired in one single trial, which makes it ideal for studying processes initiated by training, uncontaminated by prior or further trials, rehearsals, or retrievals. Biochemical events in the hippocampus related to long-term memory (LTM) formation have been extensively studied in rats using a one trial step-down IA task. DARPP-32 (dopamine and cAMP regulated phosphoprotein of Mr 32 kDa) is a cytosolic protein that is selectively enriched in medium spiny neurons in the neostriatum. It has been shown that activation of DARPP-32 and the resultant inhibition of PP-1 activity is critical for the expression of two opposing forms of brain synaptic plasticity, striatal LTD and LTP. Both forms of plasticity are also critically linked to the activation of DA receptors. It has been shown with studies in DARPP-32 KO mice an important role of this protein in mediating the effects of DA on long term changes in neuronal excitability and to our knowledge, no studies have examined the effect of IA task on DARPP-32 expression. In order to demonstrate changes in the protein expression profile we analyzed DARPP-32 levels in the striatum, prefrontal cortex (PFC), hippocampus and entorhinal cortex of Wistar rats after step-down IA learning. Our results showed that IA induced changes on DARPP-32 expression in striatum and hippocampus. DARPP-32 expression changes corroborate with changes in expression and phosphorylation of CREB, NMDA, AMPA after IA that has been reported. These changes suggest that DARPP-32 might play a central role in the IA, as previously described as an integrator of the dopaminergic signal.

Keywords

Inhibitory avoidance DARPP-32 LTD LTP Rat brain 

Abbreviations

AMPA

Alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid

CaMKII

Calcium-calmodulin-dependent protein kinase II

CREB

cAMP responsive- element-binding protein

DA

Dopamine

DARPP-32

Dopamine and cAMP regulated phosphoprotein of Mr 32 kDa

ERK

Extracellular-signal-regulated kinase

IA

Inhibitory avoidance

LTD

Long term depression

LTM

Long term memory

LTP

Long term potentiation

MAPK

Mitogen-activated protein kinase

NMDA

N-methyl-d-aspartate

PFC

Prefrontal cortex

PKA

cAMP dependent protein kinase

PKC

Protein kinase C

PKG

cGMP dependent protein kinase

PP1

Protein phosphatase 1

Ser

Serine

Thr

Threonine

Notes

Acknowledgements

This research was supported by grants from CNPq, FAPESC, Instituto Cérebro e Mente and UNESC to J. Quevedo; MVG, JQ and MAR-S are CNPq research fellows and RPS, BSM, FSC are holders of CNPq studentships and DVFR, SSV and BRS are holders of CAPES studentships. Financial support from CNPq Universal grant proc.#471837/2004-0, Programa Institutos do Milênio/CNPq/FINEP and FAPEMIG # CBB-453/04.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Daniela V. F. Rosa
    • 1
  • Renan P. Souza
    • 1
  • Bruno R. Souza
    • 1
  • Melissa M. Guimarães
    • 1
  • Daniel S. Carneiro
    • 1
  • Samira S. Valvassori
    • 2
  • Marcus V. Gomez
    • 1
  • João Quevedo
    • 2
  • Marco A. Romano-Silva
    • 1
  1. 1.Laboratório de Neurociência e Departamento de Saúde MentalFaculdade de Medicina da Universidade Federal de Minas GeraisBelo HorizonteBrazil
  2. 2.Laboratorio de Neurociências, Unidade Acadêmica de Ciências da Saúde, Programa de Pós-Graduação em Ciências da SaúdeUniversidade do Extremo Sul CatarinenseCriciumaBrazil

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