Transcription Factor GATA-3 Regulates the Transcriptional Activity of Dopamine β-Hydroxylase by Interacting with Sp1 and AP4
- First Online:
- Cite this article as:
- Hong, S.J., Choi, H.J., Hong, S. et al. Neurochem Res (2008) 33: 1821. doi:10.1007/s11064-008-9639-3
- 175 Downloads
GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at −62 to −32 bp and the other at −891 to −853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein–protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.