Neurochemical Research

, Volume 33, Issue 9, pp 1821–1831

Transcription Factor GATA-3 Regulates the Transcriptional Activity of Dopamine β-Hydroxylase by Interacting with Sp1 and AP4

  • Seok Jong Hong
  • Hyun Jin Choi
  • Sunghoi Hong
  • Youngbuhm Huh
  • Han Chae
  • Kwang-Soo Kim
Original Paper

DOI: 10.1007/s11064-008-9639-3

Cite this article as:
Hong, S.J., Choi, H.J., Hong, S. et al. Neurochem Res (2008) 33: 1821. doi:10.1007/s11064-008-9639-3

Abstract

GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine β-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at −62 to −32 bp and the other at −891 to −853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein–protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.

Keywords

GATA-3 Sp1 AP4 siRNA ChIP assay Dopamine β-hydroxylase Protein–protein interaction 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Seok Jong Hong
    • 1
  • Hyun Jin Choi
    • 1
    • 2
  • Sunghoi Hong
    • 1
  • Youngbuhm Huh
    • 1
  • Han Chae
    • 1
    • 3
  • Kwang-Soo Kim
    • 1
  1. 1.Molecular Neurobiology LaboratoryMRC215, McLean Hospital, Harvard Medical SchoolBelmontUSA
  2. 2.College of Pharmacy and Research Institute of Drug DevelopmentChonnam National UniversityGwangjuKorea
  3. 3.Pusan National University School of Oriental MedicineBusanKorea

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