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Neurochemical Research

, Volume 33, Issue 9, pp 1795–1803 | Cite as

Artepillin C Derived from Propolis Induces Neurite Outgrowth in PC12m3 Cells via ERK and p38 MAPK Pathways

  • Yoshio Kano
  • Noboru Horie
  • Shima Doi
  • Fumika Aramaki
  • Hidefumi Maeda
  • Fukumi Hiragami
  • Kenji Kawamura
  • Hirotoshi Motoda
  • Yoshihisa Koike
  • Junichi Akiyama
  • Sueo Eguchi
  • Ken Hashimoto
Original Paper

Abstract

We investigated whether artepillin C, a major component of Brazilian propolis, acts as a neurotrophic-like factor in rat PC12m3 cells, in which nerve growth factor (NGF)-induced neurite outgrowth is impaired. When cultures of PC12m3 cells were treated with artepillin C at a concentration of 20 μM, the frequency of neurite outgrowth induced by artepillin C was approximately 7-fold greater than that induced by NGF alone. Artepillin C induced-neurite outgrowth of PC12m3 cells was inhibited by the ERK inhibitor U0126 and by the p38 MAPK inhibitor SB203580. Although artepillin C-induced p38 MAPK activity was detected in PC12m3 cells, phosphorylation of ERK induced by artepillin C was not observed. On the other hand, artepillin C caused rapid activation of ERK and the time course of the activation was similar to that induced by NGF treatment in PC12 parental cells. However, NGF-induced neurite outgrowth was inhibited by artepillin C treatment. Interestingly, inhibition of ERK by U0126 completely prevented artepillin C-induced p38 MAPK phosphorylation of PC12m3 cells. These findings suggest that artepillin C-induced activation of p38 MAPK through the ERK signaling pathway is responsible for the neurite outgrowth of PC12m3 cells.

Keywords

Artepillin C NGF MAP kinase PC12m3 cells 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yoshio Kano
    • 1
  • Noboru Horie
    • 2
  • Shima Doi
    • 3
  • Fumika Aramaki
    • 3
  • Hidefumi Maeda
    • 4
  • Fukumi Hiragami
    • 5
  • Kenji Kawamura
    • 5
  • Hirotoshi Motoda
    • 5
  • Yoshihisa Koike
    • 1
  • Junichi Akiyama
    • 5
  • Sueo Eguchi
    • 5
  • Ken Hashimoto
    • 3
  1. 1.Department of Occupational Therapy, School of Health ScienceKibi International UniversityTakahashi-cityJapan
  2. 2.Department of Food Science and Nutrition, School of Human Environment ScienceMukogawa Women’s UniversityNishinomiyaJapan
  3. 3.Department of Research and DevelopmentYamada Apiculture Center, Inc.Tomata-gunJapan
  4. 4.Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental ScienceKyushu University Higashi-kuJapan
  5. 5.Department of Physical Therapy, School of Health ScienceKibi International UniversityTakahashiJapan

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