Neurochemical Research

, Volume 33, Issue 5, pp 808–813 | Cite as

Relative Expression of mRNAS Coding for Glutaminase Isoforms in CNS Tissues and CNS Tumors

  • Monika Szeliga
  • Ewa Matyja
  • Marta Obara
  • Wiesława Grajkowska
  • Tomasz Czernicki
  • Jan Albrecht
Original Paper

Abstract

Glutaminase (GA) in mammalian tissues occurs in three isoforms: LGA (liver-type), KGA (kidney-type) and GAC (a KGA variant). Our previous study showed that human malignant gliomas (WHO grades III and IV) lack expression of LGA mRNA but are enriched in GAC mRNA relative to KGA mRNA. Here we analyzed the expression of mRNAs coding for the three isoforms in the biopsy material derived from other central nervous system tumors of WHO grades I–III. Non-neoplastic resective epileptic surgery samples served as control, as did cultured rat astrocytes and neurons. The GAC mRNA/KGA mRNA expression ratio was as a rule higher in the neoplastic than in control tissues, irrespective of the cell type dominating in the tumor or tumor malignancy. LGA mRNA expression was relatively very low in cultured astrocytes, and very low to absent in astrocytoma pilocyticum, ependymoma and subependymal giant cell astrocytoma (SEGA), tumors of astrocytic origin. LGA mRNA expression was almost as high as that of KGA and GAC mRNA in cultured neurons and epileptic surgery samples which were enriched in neurons. LGA mRNA was also relatively high in ganglioglioma which contains a discernable proportion of neuronal cells, and in oligodendroglioma. The results show that low expression of LGA mRNA is a feature common to normal astrocytes and astroglia-derived tumor cells or ependymomas and can be considered as a cell-type, rather than a malignancy marker.

Keywords

Astrocytes Neurons Human brain Brain tumors Liver-type glutaminase (LGA) Kidney-type glutaminase (KGA) GAC RT-PCR 

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Monika Szeliga
    • 1
  • Ewa Matyja
    • 2
  • Marta Obara
    • 1
  • Wiesława Grajkowska
    • 3
  • Tomasz Czernicki
    • 4
  • Jan Albrecht
    • 1
  1. 1.Department of Neurotoxicology, M. Mossakowski Medical Research CenterPolish Academy of SciencesWarsawPoland
  2. 2.Department of Experimental and Clinical Neuropathology, M. Mossakowski Medical Research CenterPolish Academy of SciencesWarsawPoland
  3. 3.Department of PathologyChildren’s Memorial Health InstituteWarsawPoland
  4. 4.Department of NeurosurgeryMedical University of WarsawWarsawPoland

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