Neurochemical Research

, Volume 33, Issue 3, pp 545–550

Regulation of c-fos, c-jun and c-myc Gene Expression by Angiotensin II in Primary Cultured Rat Astrocytes: Role of ERK1/2 MAP Kinases

  • Jimmy Delaney
  • Roselynn Chiarello
  • David Villar
  • Umadevi Kandalam
  • Ana Maria Castejon
  • Michelle A. Clark
Original Paper

DOI: 10.1007/s11064-007-9474-y

Cite this article as:
Delaney, J., Chiarello, R., Villar, D. et al. Neurochem Res (2008) 33: 545. doi:10.1007/s11064-007-9474-y

Abstract

We have previously shown that angiotensin II (Ang II) stimulates astrocyte growth through activation of ERK1/2 mitogen activated protein (MAP) kinases. In the current study, we determined whether Ang II stimulates the expression of c-fos, c-jun and c-myc in brainstem astrocyte cultures. Reverse transcriptase-PCR analysis showed c-fos, c-jun, and c-myc mRNAs were induced by Ang II. The EC50 values for Ang II stimulation of c-fos, c-jun and c-myc were 1.3, 1.68 and 1.4 nM, respectively. Ang II (100 nM) induced peak stimulation for all genes by 45 min followed by a gradual decline. Inhibition of ERK1/2 by PD98059 attenuated Ang II-induced c-fos and c-myc mRNA expression (by 75% and 100%, respectively) but was ineffective in preventing Ang II induction of c-jun. These studies show for the first time in brainstem astrocytes that Ang II induces the expression of c-fos, c-myc and c-jun, and showed that ERK1/2 mediate Ang II stimulation of c-fos and c-myc. These data implicate the ERK1/2 MAP kinase pathway as a divergent point in controlling Ang II stimulation of immediate early response genes in the central nervous system.

Keywords

Brainstem Astrocytes Angiotensin II c-fos c-jun c-myc ERK1/2 MAP kinases Reverse transcriptase PCR Immediate early genes Culture 

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Jimmy Delaney
    • 1
  • Roselynn Chiarello
    • 1
  • David Villar
    • 1
  • Umadevi Kandalam
    • 1
  • Ana Maria Castejon
    • 1
  • Michelle A. Clark
    • 1
  1. 1.College of Pharmacy, Department of Pharmaceutical and Administrative Sciences, Cardiovascular and Metabolic Research UnitNova Southeastern UniversityFort LauderdaleUSA

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