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Neurochemical Research

, Volume 33, Issue 3, pp 422–429 | Cite as

Diabetic Modulation of the Temperature Kinetics Properties of Cytochrome Oxidase Activity in Rat Brain Mitochondria

  • Surendra S. Katyare
  • Samir P. Patel
  • Hiren R. Modi
Original Paper

Abstract

The effects of alloxan-diabetes and subsequent treatment with insulin on temperature kinetics properties of cytochrome oxidase activity from rat brain mitochondria were examined. The enzyme activity decreased only at the late stage of diabetes which was not normalized by insulin treatment; however at early stage of diabetes hyper-stimulation occurred. In the control animals the Arrhenius plot was chair shaped with three energies of (E1, E2 and E3) and two phase transition temperatures (Tt1 and Tt2). At early diabetic stage the Arrhenius plot became biphasic and E1 and E2 decreased; insulin treatment reversed chair-shaped pattern with increase in E2. These changes correlated with transient changes in the phospholipids profiles especially decreased acidic phospholipids. The temperature kinetics parameters were minimally affected at the late stage of diabetes or by insulin treatment. Thus at the late stage the brain tissue seems to have readjusted to its insulin homeostasis.

Keywords

Alloxan-diabetes Insulin treatment Cytochrome oxidase Temperature kinetics 

References

  1. 1.
    Kadenbach B (2003) Intrinsic and extrinsic uncoupling of oxidative phosphorylation. Biochim Biophys Acta 1604:77–94PubMedCrossRefGoogle Scholar
  2. 2.
    Richter OMH, Ludwig B (2003) Cytochrome c oxidase—structure, function, and physiology of a redox-driven molecular machine. Rev Physiol Biochem Pharmacol 147:47–74PubMedCrossRefGoogle Scholar
  3. 3.
    Wikström M (2004) Cytochrome c oxidase: 25 years of the elusive proton pump. Biochim Biophys Acta 1655:241–247PubMedCrossRefGoogle Scholar
  4. 4.
    Fry M, Green DE (1980) Cardiolipin requirement by cytochrome oxidase and the catalytic role of phospholipid. Biochem Biophys Res Commun 93:1238–1246PubMedCrossRefGoogle Scholar
  5. 5.
    Daum G (1985) Lipids of mitochondria. Biochim Biophys Acta 822:1–42PubMedGoogle Scholar
  6. 6.
    Katyare SS, Joshi MV, Fatterpaker P et al (1977) Effect of thyroid deficiency on oxidative phosphorylation in rat liver, kidney and brain mitochondria. Arch Biochem Biophys 182:155–163PubMedCrossRefGoogle Scholar
  7. 7.
    Katyare SS, Rajan RR (2005) Influence of thyroid hormone treatment on the respiratory activity of cerebral mitochondria from hypothyroid rats. A critical re-assessment. Exp Neurol 195:416–422PubMedCrossRefGoogle Scholar
  8. 8.
    Billimoria FR, Katyare SS, Patel SP (2006) Insulin status differentially affects energy transduction in cardiac mitochondria from male and female rats. Diabetes Obes Metab 8:67–74PubMedCrossRefGoogle Scholar
  9. 9.
    Katyare SS, Balasubramanian S, Parmar DV (2003) Effect of corticosterone treatment on mitochondrial oxidative energy metabolism in developing rat brain. Exp Neurol 183:241–248PubMedCrossRefGoogle Scholar
  10. 10.
    Patel MA, Katyare SS (2006) Treatment with dehydroepiandrosterone (DHEA) stimulates oxidative energy metabolism in the cerebral mitochondria. A comparative study of effects in old and young adult rats. Neurosci Lett 402:131–136PubMedCrossRefGoogle Scholar
  11. 11.
    Patel SP, Katyare SS (2005) Effect of alloxan-diabetes and subsequent treatment with insulin on lipid/phospholipid composition of rat brain microsomes and mitochondria. Neurosci Lett 399:129–134CrossRefGoogle Scholar
  12. 12.
    Murata N, Los DA (1997) Membrane fluidity and temperature perception. Plant Physiol 115:875–879PubMedGoogle Scholar
  13. 13.
    Vigh L, Maresca B, Harwood JL (1998) Does the membrane’s physical state control the expression of heat shock and other genes? Trends Biochem Sci 23:369–374PubMedCrossRefGoogle Scholar
  14. 14.
    Patel SP, Katyare SS (2006) Effect of alloxan-diabetes and subsequent treatment with insulin on kinetic properties of succinate oxidase activity from rat liver mitochondria. Z Naturforsch 61c:756–762Google Scholar
  15. 15.
    Patel SP, Katyare SS (2006) Effect of alloxan diabetes and subsequent insulin treatment on temperature kinetics properties of succinate oxidase activity in rat kidney mitochondria. J Memb Biol 213:31–37CrossRefGoogle Scholar
  16. 16.
    Patel SP, Katyare SS (2006) Insulin-status-dependent modulation of FoF1-ATPase activity in rat liver mitochondria. Lipids 41:695–703PubMedCrossRefGoogle Scholar
  17. 17.
    Patel SP, Katyare SS (2006) Insulin-status-dependent modulation of FoF1 ATPase activity in rat kidney mitochondria. Arch Physiol Biochem 112:150–157PubMedCrossRefGoogle Scholar
  18. 18.
    Patel SP, Katyare SS (2005) Differences in kinetic properties of cytochrome oxidase in mitochondria from rat tissues. A comparative study. Z Naturforsch 60c:785–791Google Scholar
  19. 19.
    Park C, Drake RL (1982) Insulin mediates the stimulation of pyruvate kinase by a dual mechanism. Biochem J 208:333–337Google Scholar
  20. 20.
    Satav JG, Katyare SS (2004) Effect of streptozotocin-induced diabetes on oxidative energy metabolism in rat liver mitochondria—A comparative study of early and late effects. Ind J Clin Biochem 19:26–36CrossRefGoogle Scholar
  21. 21.
    Katyare SS, Satav JG (2005) Effect of streptozotocin-induced diabetes on oxidative energy metabolism in rat kidney mitochondria. A comparative study of early and late effects. Diabetes Obes Metab 7:555–562PubMedCrossRefGoogle Scholar
  22. 22.
    Pandya JD, Dave KR, Katyare SS (2004) Effect of long-term aluminum feeding on lipid/phospholipid profiles of rat brain myelin. Lipids Health Dis 3:13–18PubMedCrossRefGoogle Scholar
  23. 23.
    Skipski VP, Peterson RF, Barclay M (1964) Quantitative analysis of phospholipids by thin-layer chromatography. Biochem J 90:374–378PubMedGoogle Scholar
  24. 24.
    Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468PubMedGoogle Scholar
  25. 25.
    Zlatkis A, Zak B, Boyle AJ (1953) A new method for the direct determination of serum cholesterol. J Lab Clinic Med 41:486–492Google Scholar
  26. 26.
    Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the Folin- phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  27. 27.
    Moncada S, Erusalimsky JD (2002) Does nitric oxide modulate mitochondrial energy generation and apoptosis? Nat Rev Mol Cell Biol 3:214–220PubMedCrossRefGoogle Scholar
  28. 28.
    Brown GC (1999) Nitric oxide and mitochondrial respiration. Biochim Biophys Acta 1411:351–369PubMedCrossRefGoogle Scholar
  29. 29.
    Mastrocola R, Restivo F, Vercellinatto I et al (2005) Oxidative and nitrosative stress in brain mitochondria of diabetic rats. Endocrinol 187:37–44CrossRefGoogle Scholar
  30. 30.
    Clancy RM, Levartovsky D, Leszczynska-Piziak J et al (1994) Nitric oxide reacts with intracellular glutathione and activates the hexose monophosphate shunt in human neutrophils: evidence for S-nitrosoglutathione as a bioactive intermediary. Proc Natl Acad Sci USA 91:3680–3684PubMedCrossRefGoogle Scholar
  31. 31.
    Davidson SM, Duchen MR (2006) Effects of NO on mitochondrial function in cardiomyocytes: pathophysiological relevance. Cardiovasc Res 71:10–21PubMedCrossRefGoogle Scholar
  32. 32.
    Zheng L, Du Y, Miller C et al (2007) Critical role of inducible nitric oxide synthase in degeneration of retinal capillaries in mice with streptozotocin-induced diabetes. Diabetologia [Epub ahead of print]Google Scholar
  33. 33.
    Uthra S, Raman R, Mukesh BN et al (2007) Intron 4 VNTR of endothelial nitric oxide synthase (eNOS) gene and diabetic retinopathy in type 2 patients in Southern India. Ophthalmic Genet 28:77–81PubMedCrossRefGoogle Scholar
  34. 34.
    Toda N, Nakanishi-Toda M (2007) Nitric oxide: ocular blood flow, glaucoma, and diabetic retinopathy. Prog Retin Eye Res 26:205–238PubMedCrossRefGoogle Scholar
  35. 35.
    Qian XX, Chen YM, Wu WK et al (2006) Effects of irbesartan on nitric oxide system in the heart of diabetic rats. Nan Fang Yi Ke Da Xue Xue Bao 26:1359–1362PubMedGoogle Scholar
  36. 36.
    Patel HG, Aras RV, Dave KR, Katyare SS (2000) Kinetic attributes of Na+, K+ ATPase and lipid/phospholipid profiles of rat and human erythrocyte membrane. Z Naturforsch 55c:770–777Google Scholar
  37. 37.
    Havrankova J, Schmechel D, Roth J et al (1978) Identification of insulin in rat brain. Proc Natl Acad Sci USA 75:5737–5741PubMedCrossRefGoogle Scholar
  38. 38.
    Brownlee M, Cerami A (1981) The biochemistry of the complications of diabetes mellitus. Annu Rev Biochem 50:385–432PubMedCrossRefGoogle Scholar
  39. 39.
    Sidenius P, Jakobsen J (1981) Retrograde axonal transport. A possible role in the development of neuropathy. Diabetologia 20:110–112PubMedCrossRefGoogle Scholar
  40. 40.
    Low PA, Tuck RR, Dyck PJ et al (1984) Prevention of some electrophysiologic and biochemical abnormalities with oxygen supplementation in experimental diabetic neuropathy. Proc Natl Acad Sci USA 81:6894–6898PubMedCrossRefGoogle Scholar
  41. 41.
    Pittenger G, Vinik A (2003) Nerve growth factor and diabetic neuropathy. Exp Diabesity Res 4:271–285PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Surendra S. Katyare
    • 1
  • Samir P. Patel
    • 1
  • Hiren R. Modi
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceThe Maharaja Sayajirao University of BarodaVadodaraIndia

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