Neurochemical Research

, Volume 33, Issue 1, pp 179–184 | Cite as

Glial Expression of Interleukin-18 and its Receptor After Excitotoxic Damage in the Mouse Hippocampus

  • Gye Sun Jeon
  • Sung Kyung Park
  • Sang Wook Park
  • Dong Woon Kim
  • Chun Kee Chung
  • Sa Sun Cho
Original Paper

Abstract

Interleukin (IL)-18, a member of the IL-1 cytokine family, is an important mediator of peripheral inflammation and host defence responses. However, although IL-1 is a key proinflammatory cytokine in the brain, little is known about IL-18 changes in glial cells under excitotoxic neurodegeneration. In this study, we characterized the expressions of IL-18 and IL-18 receptor (IL-18R) in kainic acid (KA)-induced excitotoxicity in mouse hippocampus by immunohistochemistry and Western blotting. IL-18 immunoreactivity was found in microglia whereas IL-18R immunoreactivity was observed in astrocytes. Levels of IL-18 and IL-18R in hippocampus homogenates increased progressively from day 1 post-KA and peaked at 3 days. This study demonstrates the cellular sources of IL-18 and IL-18R, and their temporal correlations after KA-insult, and suggests roles for IL-18 and IL-18R in glial cells in response to excitotoxic damage in the hippocampus.

Keywords

Interleukin-18 Interleukin-18 receptor Kainic acid Microglia Astrocyte 

Notes

Acknowledgments

This study was supported by the second stage Brain Korea 21 Project in 2007 and a grant (M103KV010018 04K2201 01850) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Science and Technology, the Republic of Korea. The authors thank Dr. S. Kohsaka, National Institute of Neuroscience, Japan, for the iba1 antibody.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Gye Sun Jeon
    • 1
  • Sung Kyung Park
    • 1
  • Sang Wook Park
    • 1
  • Dong Woon Kim
    • 2
  • Chun Kee Chung
    • 3
  • Sa Sun Cho
    • 1
    • 4
  1. 1.Department of AnatomySeoul National University College of MedicineSeoulSouth Korea
  2. 2.Department of AnatomyChungnam National University College of MedicineDaejeonSouth Korea
  3. 3.Department of NeurosurgerySeoul National University College of Medicine, Seoul National University HospitalSeoulSouth Korea
  4. 4.Neuroscience Research Institute, Medical Research CenterSeoul National UniversitySeoulSouth Korea

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