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Neurochemical Research

, Volume 32, Issue 8, pp 1375–1380 | Cite as

Cytoglobin Up-regulated by Hydrogen Peroxide Plays a Protective Role in Oxidative Stress

  • Dan Li
  • Xiao Qian Chen
  • Wu-Jian Li
  • Yu-Hui Yang
  • Jian-Zhi Wang
  • Albert Cheung Hoi Yu
Original Paper

Abstract

Cytoglobin (Cygb) is a recently discovered intracellular respiratory globin, which exists in all types of cells. It has been suggested that Cygb has a role in protecting cells against oxidative stress. In the present study we have tested this hypothesis. The N2a neuroblastoma cells were exposed to various kinds of insults, including hydrogen peroxide (H2O2), hypoxia, kainic acid, high extracellular CaCl2, high osmolarity, UV irradiation and heat shock. Among them, only H2O2-treatment induced a significant up-regulation of cytoglobin mRNA level. We stably transfected N2a cells with Cygb-siRNA vectors and successfully knocked down Cygb. The Cygb-siRNA could exacerbate cell death upon H2O2-treatment, as demonstrated by MTT cell viability assay. Thus, Cygb in neuronal cells might be specifically induced under oxidative stress to protect them from death.

Keywords

Cytoglobin Oxidative stress N2a neuroblastoma cell siRNA Hydrogen peroxide 

Notes

Acknowledgments

The authors thank Miss Nelly Lam from Hong Kong DNA Chips Limited for assistance in preparation of this article. This work was supported by grants from the National Natural Science Foundation of China (30470539 and 30570555) and a grant of “Program for New Century Excellent Talents in University” to X.Q. Chen.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Dan Li
    • 1
  • Xiao Qian Chen
    • 1
  • Wu-Jian Li
    • 1
  • Yu-Hui Yang
    • 1
  • Jian-Zhi Wang
    • 1
  • Albert Cheung Hoi Yu
    • 2
    • 3
    • 4
  1. 1.Department of Pathophysiology and Hubei Provincial Key Laboratory of Neurological Diseases, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Neuroscience Research InstitutePeking UniversityBeijingChina
  3. 3.Key Laboratory of Neuroscience (PKU), Ministry of EducationBeijingChina
  4. 4.Department of NeurobiologyPeking University Health Science CenterBeijingChina

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