Neurochemical Research

, Volume 32, Issue 7, pp 1120–1128 | Cite as

Differential Expression of Cerebellar Metabotropic Glutamate Receptors mGLUR2/3 and mGLUR4a after the Administration of a Convulsant Drug and the Adenosine Analogue Cyclopentyladenosine

  • Elena Silvia Girardi
  • Juan Canitrot
  • Marta Antonelli
  • Nélida N. González
  • Héctor Coirini
Original Paper
First Online:
Received:
Accepted:

Abstract

Metabotropic glutamate receptors (mGluR) play a role in synaptic transmission, neuronal modulation and plasticity but their action in epileptic activity is still controversial. On the other hand adenosine acts as a neuromodulator with endogenous anticonvulsive properties. Since cerebellum from epileptic patients has shown neuronal damage, sometimes associated with Purkinje cells loss, we have explored the effect of repetitive seizures on two types of mGluR in the cerebellum. Seizures were induced by the convulsant drug 3-mercaptopropionic acid (MP) and the effect of the adenosine analogue cyclopentyladenosine (CPA) alone or before MP administration (CPA+MP) were also evaluated. The expression of the receptors subtypes 2/3 (mGluR2/3) and 4a (mGluR4a) was assessed by immunocitochemistry. Granular cell layer was labeled with mGluR2/3 antibody and increased immunoreactivity was observed after MP (60%), CPA (53%) and CPA + MP (85%) treatments. Control cerebellum slices showed mGluR4a reactivity around Purkinje cells, while MP, CPA and CPA+MP treatment decreased this immunostaining. Repetitive administration of MP and CPA induces an increased cerebellar mGluR2/3 and a decreased mGluR4a immunostaining, suggesting a distinct participation of both receptors that may be related to the type of cell involved. A protective action and /or an apoptotic effect may not be discarded. CPA repetitive administration although increase seizure latency, cannot prevent seizure activity.

Keywords

Metabotropic receptors mGluR2/3 mGluR4a Cerebellum Epilepsy Adenosine Seizure Convulsant drug Cyclopentyladenosine 3-Mercaptopropionic acid 
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Notes

Acknowledgment

This work was supported by grants of UBACYT M033 and M020 from Universidad de Buenos Aires, and CONICET (PIP N° 02267, 5798) from the Consejo Nacional de Investigaciones Científicas y Técnicas. We are grateful to Mrs Susana Buglione for her collaboration in the bibliographic management of the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Elena Silvia Girardi
    • 1
  • Juan Canitrot
    • 1
  • Marta Antonelli
    • 2
  • Nélida N. González
    • 1
  • Héctor Coirini
    • 3
  1. 1.Instituto de Biología Celular y Neurociencia “Prof. Eduardo De Robertis” Facultad de MedicinaUniversidad de Buenos Aires. Buenos Aires. Argentina y Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina
  2. 2.IQUIFIB, UBA-CONICETBuenos AiresArgentina
  3. 3.Department of Human BiochemistryIBYME-CONICET, Facultad de Medicina. UBABuenos AiresArgentina

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