Neurochemical Research

, Volume 32, Issue 2, pp 213–228

White Matter Rafting––Membrane Microdomains in Myelin

Original Paper

Abstract

The myelin membrane comprises a plethora of regions that are compositionally, ultrastructurally, and functionally distinct. Biochemical dissection of oligodendrocytes, Schwann cells, and central and peripheral nervous system myelin by means such as cold-detergent extraction and differential fractionation has led to the identification of a variety of detergent-resistant membrane assemblies, some of which represent putative signalling platforms. We review here the different microdomains that have hitherto been identified in the myelin membrane, particularly lipid rafts, caveolae, and cellular junctions such as the tight junctions that are found in the radial component of the CNS myelin sheath.

Keywords

Lipid rafts Detergent-resistant membranes Cholesterol Caveolae Myelin Oligodendrocytes Radial component Cellular junctions 

Abbreviations

BMP

bone morphogenetic protein

CEM

caveolin-enriched microdomain

CHAPS

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulphonate

CGT

ceramide galactosyl transferase

CNP

2′3′-cyclic nucleotide 3′phosphodiesterase

CNS

central nervous system

Cx

connexin

DM20

deletion mutant of PLP

DRM

detergent-resistant membrane

EAE

experimental autoimmune encephalomyelitis

eNOS

endothelial nitric oxide synthase

EPR

electron paramagnetic resonance

FRET

fluorescence resonance energy transfer

GPI

glycosylphosphatidylinositol

ld

liquid-disordered

lo

liquid-ordered

MAL

myelin and lymphocyte protein

MAG

myelin-associated glycoprotein

MAPK

mitogen-activated protein kinase

MBP

myelin basic protein

MEK

MAP kinase kinase

mER

membrane-bound estrogen receptor

MOG

myelin/oligodendrocyte glycoprotein

MOBP

myelin-associated oligodendrocyte basic protein

MOSP

myelin/oligodendrocyte specific protein

MS

multiple sclerosis

NF155

neurofascin isoform of 155 kDa

NMR

nuclear magnetic resonance

OL

oligodendrocyte

OMgp

oligodendrocyte-myelin glycoprotein

OSP

oligodendrocyte-specific protein

p75NTR

p75 neurotrophin receptor

PDGF

platelet-derived growth factor

PI3K

phosphatidylinositol-3 kinase

PLP

proteolipid protein

PMP22

peripheral myelin protein 22

PNS

peripheral nervous system

SC

Schwann cell

TM4SF

transmembrane 4 superfamily

TX-100

Triton X-100

ZO-1

zonula occludens-1

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Department of Molecular and Cellular Biology, and Biophysics Interdepartmental GroupUniversity of GuelphGuelphCanada
  2. 2.Department of ChemistryWilfrid Laurier UniversityWaterlooCanada

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