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Differential Effects of Polyunsaturated Fatty Acids on Membrane Capacitance and Exocytosis in Rat Pheochromocytoma-12 Cells

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The fusion of synaptic vesicles with the plasma membrane during exocytosis can be recorded by membrane capacitance measurements under voltage-clamp conditions. These measurements enable high time-resolution quantitation of exocytosis. The present study was carried out using the above technique to elucidate the effects of various polyunsaturated fatty acids on exocytosis in a neuroendocrine cell, the rat pheochromocytoma-12 (PC12) cell. External application of eicosapentaenoic acid and arachidonic acid resulted in an increase in capacitance of PC12 cells, indicating fusion of secretory vesicles with cell membranes and exocytosis. In contrast, docosahexaenoic acid, linoleic acid, oleic acid, and vehicle control had no significant effect on capacitance. The above findings show differential effects of polyunsaturated fatty acids on exocytosis in PC12 cells. It is postulated that besides arachidonic acid, eicosapentaenoic acid could also play an important role in exocytosis and neurotransmitter release, in neurons and hormone-secreting cells.

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Authors and Affiliations

  1. Institutes of Microelectronics, A*Star, Singapore

    Wee-Liat Ong

  2. Department of Acupuncture, General Hospital of the People’s Liberation Army, Beijing, 100853, PR China

    Bin Jiang

  3. Departments of Oral and Maxillofacial Surgery, National University of Singapore, Singapore

    Ning Tang, Su-Fung Ling & Jin-Fei Yeo

  4. Institute of Molecular and Cell Biology, A*Star, Singapore

    Shunhui Wei

  5. Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH, 43210, USA

    Akhlaq A. Farooqui

  6. Department of Anatomy, National University of Singapore, 119260, Singapore

    Wei-Yi Ong

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  1. Wee-Liat Ong
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Wee-Liat Ong and Bin Jiang - These authors contributed equally to the work.

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Ong, WL., Jiang, B., Tang, N. et al. Differential Effects of Polyunsaturated Fatty Acids on Membrane Capacitance and Exocytosis in Rat Pheochromocytoma-12 Cells. Neurochem Res 31, 41–48 (2006). https://doi.org/10.1007/s11064-005-9002-x

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