Neurochemical Research

, Volume 30, Issue 4, pp 583–589 | Cite as

Neuroprotection and Protein Damage Prevention by Estradiol Replacement in Rat Hippocampal Slices Exposed to Oxygen-Glucose Deprivation

  • Helena Cimarosti
  • Ionara R. Siqueira
  • Lauren L. Zamin
  • Melissa Nassif
  • Rodrigo Balk
  • Rudimar Frozza
  • Carla Dalmaz
  • Carlos Alexandre Netto
  • Christianne Salbego
Article

ABSTRACT

Here we investigated the effects of estradiol replacement in ovariectomized female rats using hippocampal slices exposed to oxygen-glucose deprivation (OGD). OGD induced lactate dehydrogenase (LDH) release to the incubation medium, what was assumed as a parameter of cellular death. In the estradiol-treated group the LDH release was markedly decreased by 23% as compared to the vehicle-treated group. In attempt to study a possible mechanism by which estradiol acts, we investigated some parameters of oxidative stress. In both vehicle-treated and estradiol-treated groups, OGD significantly increased the free radical production by 34% and 16%, respectively, although no significant differences on total antioxidant capacity were observed. Interestingly, estradiol replacement prevented the significant reduction in tryptophan and tyrosine contents caused by OGD observed in vehicle-treated animals. Our results show that estradiol replacement in ovariectomized female rats decreases cellular susceptibility to an ischemic-like injury and suggest a role for the hormone on protein damage prevention.

Keywords

Cerebral ischemia estradiol replacement hippocampal slices neuroprotection oxidative stress protein damage 

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Helena Cimarosti
    • 1
  • Ionara R. Siqueira
    • 2
  • Lauren L. Zamin
    • 1
  • Melissa Nassif
    • 1
  • Rodrigo Balk
    • 1
  • Rudimar Frozza
    • 1
  • Carla Dalmaz
    • 1
  • Carlos Alexandre Netto
    • 1
  • Christianne Salbego
    • 1
  1. 1.Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde UFRGS Porto AlegreBrazil
  2. 2.Centro Universitário UNIVATES LajeadoBrazil

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