Effects of Olive Leaf Extract on Myoclonic and Tonico-Clonic Seizures in Mice

  • R. Shahabi
  • F. Ramezani
  • F. Nasirinezhad
  • B. Shahabi
  • M. RostampourEmail author

Effects of a hydroalcoholic olive leaf extract (OLE) on pentylenetetrazol (PTZ, 80 mg/kg)-induced seizures in mice were examined. As was found, the OLE significantly increased the delay of PTZinitiated myoclonic seizures when injected i.p. in a dose of 1000 mg/kg and also significantly delayed the initiation of tonico-clonic seizures in doses from 250 to 1000 mg/kg. The effects of the OLE on clonico-tonic seizures were more intense than those on myoclonic seizures. The OLE also significantly reduced the mortality rate within a 24-h observation period after PTZ injections. At 125 and 1000 mg/kg doses, 2 and 7 animals of 8 individuals in the OLE-treated groups survived, while all 8 mice died during 24 h in the control group.


olive leaf extract (OLE) pentylenetetrazol mice myoclonic seizures tonico-clonic seizures mortality rate 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • R. Shahabi
    • 1
  • F. Ramezani
    • 2
  • F. Nasirinezhad
    • 3
  • B. Shahabi
    • 4
  • M. Rostampour
    • 5
    Email author
  1. 1.Department of PhysiologyIran University of Medical SciencesTehranIran
  2. 2.Department of Physiology, Physiology Research CenterFaculty of MedicineTehranIran
  3. 3.Physiology Research Center and Department of PhysiologyTehranIran
  4. 4.Islamic Azad University Science and Research Branch of TehranTehranIran
  5. 5.Cellular and Molecular Research Center and Department of PhysiologyGilan University of Medical SciencesRashtIran

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