Inhibition of glioma growth by a GOLPH3 siRNA-loaded cationic liposomes
GOLPH3 has been shown to be involved in glioma proliferation. In this study, we aimed to demonstrate that GOLPH3 can serve as a target for glioma gene therapy.
During the experiment, cationic liposomes with angiopep-2 (A2-CL) were used to deliver siGOLPH3 crossing the blood–brain barrier and reaching the glioma.
At the cellular level, the A2-CL/siGOLPH3 could silence GOLPH3 and then effectively inhibited the proliferation of cells. In vivo experiments, using U87-GFP-Luci-bearing BALB/c mouse models, we demonstrated that A2-CL could deliver GOLPH3-siRNA specifically to glioma and effectively inhibit glioma growth.
This study shows that GOLPH3 has great potential as a target for the gene therapy of glioma and is of great value in precise medical applications.
KeywordsGlioma GOLPH3 RNAi Angiopep-2 Liposome
This work was financially supported by National Natural Science Foundation of China (Grants No. 81772665, 81502153, 81472345, 81302175), Natural Science Foundation of Jiangsu Province (Grants No. BK20150221), China Postdoctoral Science Foundation funded project (Grants No. 2016M591926; 2017T100409), Jiangsu Province, Key Research & Development Plan of Jiangsu Province (No. BE2016646), Jiangsu provincial Commission of Health and Family Planning (Grants No. Q201608), Jiangsu Provincial Medical Youth Talent (QNRC2016786), and Postgraduate Research & Practice Innovation Program of Jiangsu Province (Grants No. SJCX17_0551).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent was obtained from all individual participants included in the study.
Research involving human or animal rights
This article does not contain any studies with human participants performed by any of the authors. This article contains studies with animals performed by authors and all applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
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