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Journal of Neuro-Oncology

, Volume 140, Issue 2, pp 281–288 | Cite as

Long non-coding RNA MEG3 regulates proliferation, apoptosis, and autophagy and is associated with prognosis in glioma

  • Haikang Zhao
  • Xin Wang
  • Xiaoyun Feng
  • Xiaoqiang Li
  • Li Pan
  • Jianrong Liu
  • Fenglu Wang
  • Zhihai Yuan
  • Lei Yang
  • Jun Yu
  • Rujuan Su
  • Yuelin Zhang
  • Lianting Ma
Clinical Study

Abstract

Purpose

Accumulating evidence indicates that dysregulated long noncoding RNAs (lncRNAs) play critical roles in tumorigenesis and cancer progression. LncRNA-maternally expressed gene 3 (MEG3) has been shown to be involved in the initiation and development of several cancers, including glioma. However, the clinical prognostic value of MEG3 in glioma has not yet been fully elucidated.

Methods

The expression levels of MEG3 were detected in 79 glioma tissues and adjacent normal brain tissues, as well as, glioma cells and normal human astrocytes by qRT-PCR. Kaplan–Meier and Cox regression methods were utilized for the survival analysis. MTT assay, flow cytometry, and immunofluorescence assay were carried out to detect the impact of MEG3 on glioma cell proliferation, apoptosis, and autophagy.

Result

The current results showed that MEG3 expression was significantly downregulated in glioma tissues and cell line and negatively correlated with WHO grade in glioma patients. Low MEG3 expression was significantly associated with the advanced WHO grade, low Karnofsky performance score (KPS), IDH wild-type, and tumor recurrence. Patients displaying a low expression of MEG3 contributed to poor overall survival. The downregulated level of MEG3, advanced WHO grade, low KPS, IDH wild-type, and tumor recurrence were independent poor prognostic indicators in glioma patients. The in vitro experiments demonstrated that the MEG3 overexpression remarkably suppressed the proliferation while facilitating apoptosis and autophagy in glioma cells.

Conclusions

These findings indicated a critical role of MEG3 in glioma cell proliferation, apoptosis, and autophagy. Also, the gene was found to be significantly associated with the prognosis in glioma patients. Thus, it might provide a new target for predicting prognosis and therapeutic intervention in glioma.

Keywords

LncRNAs MEG3 Proliferation Apoptosis Autophagy Prognosis Glioma 

Notes

Acknowledgements

We thank Professor Li for assistance in language editing.

Funding

This work was supported by the National Natural Science Funds (No. 81470438) and Fund for the Development of Key Subjects from Xi’an Medical University.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethics approval

The study was approved by the Research Ethics Committee of Wuhan General Hospital of PLA, and it complied with the Declaration of Helsinki. All procedures performed in this study were in accordance with the 1964 Helsinki Declaration and its later amendments.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeurosurgeryWuhan General Hospital of PLAWuhanChina
  2. 2.Department of Neurosurgery, The Second Affiliated HospitalXi’an Medical UniversityXi’anChina
  3. 3.Department of Neurosurgery, Tangdu HospitalFourth Military Medical UniversityXi’anChina
  4. 4.Experimental Center, The Second Affiliated HospitalXi’an Medical UniversityXi’anChina
  5. 5.Department of OncologyFirst Affiliated Hospital of Medical College of Xi’an Jiaotong UniversityXi’anChina
  6. 6.Shaanxi Key Laboratory of Preventinon and Therapy of Cerebral DiseaseXi’an Medical UniversityXi’anChina

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