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Journal of Neuro-Oncology

, Volume 140, Issue 3, pp 539–546 | Cite as

Possible effects of radiofrequency electromagnetic fields on in vivo C6 brain tumors in Wistar rats

  • Nihal S. Ouadah
  • Anthony Lecomte
  • Franck Robidel
  • Ann Olsson
  • Isabelle Deltour
  • Joachim Schüz
  • Kelly Blazy
  • Anne-Sophie VillégierEmail author
Laboratory Investigation

Abstract

Purpose

Glioblastoma is a malignant brain tumor which has one of the poorest prognosis. It is not clear if toxic environmental factors can influence its aggressiveness. Recently, it was suggested that brain cancer patients with heavy cell phone use showed reduced survival. Here we aimed to assess the effect of controlled brain averaged specific absorption rate (BASAR) from heavy use of cell phone radiofrequency electromagnetic fields (RF–EMF) on in vivo C6 brain tumors in Wistar rats.

Methods

C6 cells grafted male rats were exposed to GSM 900 MHz signal at environmental BASAR, 0 (sham), 0.25 or 0.5 W/kg (5 days a week, 45 min a day in restraint), or were cage controls (no restraint). At death, tumor volume and immunohistochemistry for CD31, cleaved caspase (CC) 3 and Ki67 were assessed to examine vascularization, apoptosis and cellular divisions, respectively. Moreover, immune cell invasion, necrosis and mitotic index were determined.

Results

Results showed no BASAR effect on survival (31 days post-graft median), tumor volume, mitotic index, vascularization, infiltration, necrosis or cell division. However, results suggested a BASAR-dependent reduction of immune cell invasion and apoptosis.

Conclusions

Our data suggested an action of RF–EMF by reducing immune cell invasion and glioblastoma cell apoptosis, at probably too low amplitude to impact survival. Further replication studies are needed to confirm these observations.

Keywords

Glioblastoma Radiofrequencies Apoptosis Immune cell invasion Cell phone 

Notes

Acknowledgements

This work was financed by the French National Research Program for Environmental and Occupational Health of ANSES [2014/2 RF/002] and French Ministry of Ecology [Program 190].

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Institut national de l’environnement industriel et des risques (INERIS), Unité de Toxicologie Expérimentale, Parc Technologique ALATAVerneuil-en-HalatteFrance
  2. 2.PERITOX-INERIS laboratoryCURS, Picardie University Jules Verne, CHU SudAmiensFrance
  3. 3.Section of Environment and RadiationInternational Agency for Research on Cancer (IARC)LyonFrance

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