Journal of Neuro-Oncology

, Volume 128, Issue 1, pp 93–100 | Cite as

Tumor DNA in cerebral spinal fluid reflects clinical course in a patient with melanoma leptomeningeal brain metastases

  • Yingmei Li
  • Wenying Pan
  • Ian D. Connolly
  • Sunil Reddy
  • Seema Nagpal
  • Stephen Quake
  • Melanie Hayden Gephart
Clinical Study

Abstract

Cerebral spinal fluid (CSF) from brain tumor patients contains tumor cellular and cell-free DNA (cfDNA), which provides a less-invasive and routinely accessible method to obtain tumor genomic information. In this report, we used droplet digital PCR to test mutant tumor DNA in CSF of a patient to monitor the treatment response of metastatic melanoma leptomeningeal disease (LMD). The primary melanoma was known to have a BRAFV600E mutation, and the patient was treated with whole brain radiotherapy and BRAF inhibitors. We collected 9 CSF samples over 6 months. The mutant cfDNA fraction gradually decreased from 53 % (time of diagnosis) to 0 (time of symptom alleviation) over the first 6 time points. Three months after clinical improvement, the patient returned with severe symptoms and the mutant cfDNA was again detected in CSF at high levels. The mutant DNA fraction corresponded well with the patient’s clinical response. We used whole exome sequencing to examine the mutation profiles of the LMD tumor DNA in CSF before therapeutic response and after disease relapse, and discovered a canonical cancer mutation PTENR130* at both time points. The cellular and cfDNA revealed similar mutation profiles, suggesting cfDNA is representative of LMD cells. This study demonstrates the potential of using cellular or cfDNA in CSF to monitor treatment response for LMD.

Keywords

Brain Tumor Cell-free DNA Cerebral spinal fluid Droplet digital PCR Exome sequencing Leptomeningeal disease Melanoma 

Supplementary material

11060_2016_2081_MOESM1_ESM.docx (2.5 mb)
Supplementary material 1 (DOCX 2527 kb)
11060_2016_2081_MOESM2_ESM.xlsx (73 kb)
Supplementary material 2 (XLSX 73 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yingmei Li
    • 1
  • Wenying Pan
    • 3
  • Ian D. Connolly
    • 1
  • Sunil Reddy
    • 4
  • Seema Nagpal
    • 5
  • Stephen Quake
    • 3
    • 6
  • Melanie Hayden Gephart
    • 2
  1. 1.Department of NeurosurgeryStanford UniversityStanfordUSA
  2. 2.Department of NeurosurgeryStanford UniversityStanfordUSA
  3. 3.Department of Bioengineering, James H. Clark CenterStanford UniversityStanfordUSA
  4. 4.Department of Medicine, Cancer CenterStanford UniversityStanfordUSA
  5. 5.Department of Neurology, Cancer CenterStanford UniversityStanfordUSA
  6. 6.Howard Hughes Medical InstituteChevy ChaseUSA

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