Abstract
5-aminolevulinic acid (5-ALA) introduction in the surgical management of Glioblastoma (GBM) enables the intra-operatively identification of cancer cells in the mass by means of fluorescence. Here, we analyzed the phenotype of GBM cells isolated from distinct tumour areas determined by 5-ALA (tumour core, 5-ALA intense and vague layers) and the potency of 5-ALA labelling in identifying GBM cells and cancer stem cells (CSCs) in the mass. 5-ALA identified distinct layers in the mass, with less differentiated cells residing in the core of the tumour. 5-ALA was able to stain up to 68.5 % of CD133+ cells in the 5-ALA intense layer and, although 5-ALA+ cells retrieved from different tumour areas contained a similar proportion of CD133+ cells (range 27.5–35.6 %), those from the vague layer displayed the lowest ability to self-renew. In conclusion, our data demonstrate that a substantial amount of GBM cells and CSCs in the mass are able to avoid 5-ALA labelling and support the presence of heterogenic CSC populations in the GBM mass.
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Acknowledgments
This work was supported by Grants from Fondazione Città della Speranza (to CF and GB), FIRB project 2011 #RBAP11TF7Z_004 (to GB) and University of Padova—Progetto Giovani Studiosi 2010 Grant (to LP). ER is supported by a fellowship from Italian Association for Cancer Research (AIRC).
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Elena Rampazzo and Alessandro Della Puppa contributed equally to this study.
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Rampazzo, E., Della Puppa, A., Frasson, C. et al. Phenotypic and functional characterization of Glioblastoma cancer stem cells identified trough 5-aminolevulinic acid-assisted surgery. J Neurooncol 116, 505–513 (2014). https://doi.org/10.1007/s11060-013-1348-3
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DOI: https://doi.org/10.1007/s11060-013-1348-3